ArticlePloS one2012
The transcriptome of Lutzomyia longipalpis (Diptera: Psychodidae) male reproductive organs.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 37 citations in OpenAlex.
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- Molecular mechanisms underlying milk production and viviparity in the cockroach, Diploptera punctata.Insect biochemistry and molecular biology · 2020Article
- Serpins in arthropod biology.Seminars in cell & developmental biology · 2017Review
- Comparative genomics identifies male accessory gland proteins in fiveWellcome open research · 2017Article
- Positive selection and comparative molecular evolution of reproductive proteins from New Zealand tree weta (Orthoptera, Hemideina).PloS one · 2017Article
- The Lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication.Parasites & vectors · 2016Review
- Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction.Scientific reports · 2015Article
- We can't all be supermodels: the value of comparative transcriptomics to the study of non-model insects.Insect molecular biology · 2015Review
- Identification and characterization of seminal fluid proteins in the Asian tiger mosquito, Aedes albopictus.PLoS neglected tropical diseases · 2014Article
- STINGRAY: system for integrated genomic resources and analysis.BMC research notes · 2014Article
- Transcriptome exploration of the sex pheromone gland of Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae).Parasites & vectors · 2013Article
- Identification and function of proteolysis regulators in seminal fluid.Molecular reproduction and development · 2013Review
- Molecular analysis of an odorant-binding protein gene in two sympatric species of Lutzomyia longipalpis s.l.Memorias do Instituto Oswaldo Cruz · 2013Article
- Circadian rhythms in insect disease vectors.Memorias do Instituto Oswaldo Cruz · 2013Review
- Article
- First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis.PloS one · 2013Article
- Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundIt has been suggested that genes involved in the reproductive biology of insect disease vectors are potential targets for future alternative methods of control. Little is known about the molecular biology of reproduction in phlebotomine sand flies and there is no information available concerning genes that are expressed in male reproductive organs of Lutzomyia longipalpis, the main vector of American visceral leishmaniasis and a species complex. METHODS/PRINCIPAL
findingsWe generated 2678 high quality ESTs ("Expressed Sequence Tags") of L. longipalpis male reproductive organs that were grouped in 1391 non-redundant sequences (1136 singlets and 255 clusters). BLAST analysis revealed that only 57% of these sequences share similarity with a L. longipalpis female EST database. Although no more than 36% of the non-redundant sequences showed similarity to protein sequences deposited in databases, more than half of them presented the best-match hits with mosquito genes. Gene ontology analysis identified subsets of genes involved in biological processes such as protein biosynthesis and DNA replication, which are probably associated with spermatogenesis. A number of non-redundant sequences were also identified as putative male reproductive gland proteins (mRGPs), also known as male accessory gland protein genes (Acps).
conclusionsThe transcriptome analysis of L. longipalpis male reproductive organs is one step further in the study of the molecular basis of the reproductive biology of this important species complex. It has allowed the identification of genes potentially involved in spermatogenesis as well as putative mRGPs sequences, which have been studied in many insect species because of their effects on female post-mating behavior and physiology and their potential role in sexual selection and speciation. These data open a number of new avenues for further research in the molecular and evolutionary reproductive biology of sand flies.
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