ArticleThe EMBO journal1990
Specific association of STOP protein with microtubules in vitro and with stable microtubules in mitotic spindles of cultured cells.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Microtubule-Associated Proteins: From Dynamic Regulation of Microtubules to Cellular Architecture.Cells · 2026Review
- Multi-omics Investigation of Freeze Tolerance in the Amur Sleeper, an Aquatic Ectothermic Vertebrate.Molecular biology and evolution · 2023Article
- Calcium entry units (CEUs): perspectives in skeletal muscle function and disease.Journal of muscle research and cell motility · 2021Article
- Beyond Neuronal Microtubule Stabilization: MAP6 and CRMPS, Two Converging Stories.Frontiers in molecular neuroscience · 2021Article
- Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction.Skeletal muscle · 2018Article
- Parvulin 17-catalyzed Tubulin Polymerization Is Regulated by Calmodulin in a Calcium-dependent Manner.The Journal of biological chemistry · 2015Article
- Structural basis for the association of MAP6 protein with microtubules and its regulation by calmodulin.The Journal of biological chemistry · 2013Article
- Herpes simplex virus type 1 tegument protein VP22 induces the stabilization and hyperacetylation of microtubules.Journal of virology · 1998Article
- Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- A 60-kDa plant microtubule-associated protein promotes the growth and stabilization of neurotubules in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Cloning, expression, and properties of the microtubule-stabilizing protein STOP.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Binding of E-MAP-115 to microtubules is regulated by cell cycle-dependent phosphorylation.The Journal of cell biology · 1995Article
- Microtubule dependency of p34cdc2 inactivation and mitotic exit in mammalian cells.The Journal of cell biology · 1994Article
- Intrinsic microtubule stability in interphase cells.The Journal of cell biology · 1994Article
- The plus ends of stable microtubules are the exclusive nucleating structures for microtubules in the axon.The Journal of cell biology · 1992Article
- Mitotic motors.The Journal of cell biology · 1991Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
STOP (Stable Tubule Only Polypeptide) is a neuronal microtubule associated protein of 145 kd that stabilizes microtubules indefinitely to in vitro disassembly induced by cold temperature, millimolar calcium or by drugs. We have produced monoclonal antibodies against STOP. Using an antibody affinity column, we have produced a homogeneously pure 145 kd protein which has STOP activity as defined by its ability to induce cold stability and resistance to dilution induced disassembly in microtubules in vitro. Western blot analysis, using a specific monoclonal antibody, demonstrates that STOP recycles quantitatively with microtubules through three assembly cycles in vitro. Immunofluorescence analysis demonstrates that STOP is specifically associated with microtubules of mitotic spindles in neuronal cells. Further, and most interestingly, STOP at physiological temperature appears to be preferentially distributed on the distinct microtubule subpopulations that display cold stability; kinetochore-to-pole microtubules and telophase midbody microtubules. The observed distribution suggests that STOP induces the observed cold stability of these microtubule subpopulations in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.