ArticleThe Journal of biological chemistry2012
Activation of archaeal transcription mediated by recruitment of transcription factor B.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- High hydrostatic pressure promotes gene transcription via a cystathionine-β-synthase domain-containing protein in the hyperthermophilic archaeon Pyrococcus yayanosii.Nucleic acids research · 2025Article
- The transcriptional regulator EarA and intergenic terminator sequences modulate archaellation inFrontiers in microbiology · 2023Article
- Promoter-proximal elongation regulates transcription in archaea.Nature communications · 2021Article
- Archaeal transcription.Transcription · 2020Review
- Evolution of Regulated Transcription.Cells · 2020Review
- CopR, a Global Regulator of Transcription to Maintain Copper Homeostasis inFrontiers in microbiology · 2020Article
- The Transcriptional Regulator TFB-RF1 Activates Transcription of a Putative ABC Transporter inFrontiers in microbiology · 2018Article
- Extreme Deviations from Expected Evolutionary Rates in Archaeal Protein Families.Genome biology and evolution · 2017Article
- Structure and mechanisms of viral transcription factors in archaea.Extremophiles : life under extreme conditions · 2017Review
- Gene regulation of two ferredoxin:NADPExtremophiles : life under extreme conditions · 2017Article
- Same same but different: The evolution of TBP in archaea and their eukaryotic offspring.Transcription · 2017Article
- A conserved hexanucleotide motif is important in UV-inducible promoters in Sulfolobus acidocaldarius.Microbiology (Reading, England) · 2017Article
- Bypassing the Need for the Transcriptional Activator EarA through a Spontaneous Deletion in the BRE Portion of theFrontiers in microbiology · 2017Article
- Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.Nature communications · 2016Article
- Transcription Regulation in Archaea.Journal of bacteriology · 2016Review
- Genome-wide binding analysis of the transcriptional regulator TrmBL1 in Pyrococcus furiosus.BMC genomics · 2016Article
- The interplay between nucleoid organization and transcription in archaeal genomes.Nature reviews. Microbiology · 2015Review
- The TrmB family: a versatile group of transcriptional regulators in Archaea.Extremophiles : life under extreme conditions · 2014Review
- Article
- The Sulfolobus initiator element is an important contributor to promoter strength.Journal of bacteriology · 2013Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Archaeal promoters consist of a TATA box and a purine-rich adjacent upstream sequence (transcription factor B (TFB)-responsive element (BRE)), which are bound by the transcription factors TATA box-binding protein (TBP) and TFB. Currently, only a few activators of archaeal transcription have been experimentally characterized. The best studied activator, Ptr2, mediates activation by recruitment of TBP. Here, we present a detailed biochemical analysis of an archaeal transcriptional activator, PF1088, which was identified in Pyrococcus furiosus by a bioinformatic approach. Operon predictions suggested that an upstream gene, pf1089, is polycistronically transcribed with pf1088. We demonstrate that PF1088 stimulates in vitro transcription by up to 7-fold when the pf1089 promoter is used as a template. By DNase I and hydroxyl radical footprinting experiments, we show that the binding site of PF1088 is located directly upstream of the BRE of pf1089. Mutational analysis indicated that activation requires the presence of the binding site for PF1088. Furthermore, we show that activation of transcription by PF1088 is dependent upon the presence of an imperfect BRE and is abolished when the pf1089 BRE is replaced with a BRE from a strong archaeal promoter. Gel shift experiments showed that TFB recruitment to the pf1089 operon is stimulated by PF1088, and TFB seems to stabilize PF1088 operator binding even in the absence of TBP. Taken together, these results represent the first biochemical evidence for a transcriptional activator working as a TFB recruitment factor in Archaea, for which the designation TFB-RF1 is suggested.
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