ArticleAmerican journal of physiology. Renal physiology2012
ATP mediates flow-induced NO production in thick ascending limbs.
Article in American journal of physiology. Renal physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 32 citations in OpenAlex.
- Mechanisms of decreased tubular flow-induced nitric oxide in Dahl salt-sensitive rat thick ascending limbs.American journal of physiology. Renal physiology · 2021Article
- Sensing of tubular flow and renal electrolyte transport.Nature reviews. Nephrology · 2020Review
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.Physiological reviews · 2019Review
- Effect of spatial heterogeneity and colocalization of eNOS and capacitative calcium entry channels on shear stress-induced NO production by endothelial cells: A modeling approach.Cellular and molecular bioengineering · 2018Article
- Nitric oxide production by glomerular podocytes.Nitric oxide : biology and chemistry · 2018Article
- Acute In Vivo Analysis of ATP Release in Rat Kidneys in Response to Changes of Renal Perfusion Pressure.Journal of the American Heart Association · 2017Article
- Role of renal transporters and novel regulatory interactions in the TAL that control blood pressure.Physiological genomics · 2017Review
- Cholesterol Enrichment Impairs Capacitative Calcium Entry, eNOS Phosphorylation & Shear Stress-Induced NO Production.Cellular and molecular bioengineering · 2017Article
- Angiotensin II stimulates superoxide production by nitric oxide synthase in thick ascending limbs.Physiological reports · 2016Article
- Mathematical model for shear stress dependent NO and adenine nucleotide production from endothelial cells.Nitric oxide : biology and chemistry · 2016Article
- Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organism.Eukaryotic cell · 2015Article
- TRPV4 mediates flow-induced increases in intracellular Ca in medullary thick ascending limbs.Acta physiologica (Oxford, England) · 2015Article
- Integrated control of Na transport along the nephron.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- Endogenous flow-induced nitric oxide reduces superoxide-stimulated Na/H exchange activity via PKG in thick ascending limbs.American journal of physiology. Renal physiology · 2015Article
- Angiotensin II-induced hypertension blunts thick ascending limb NO production by reducing NO synthase 3 expression and enhancing threonine 495 phosphorylation.American journal of physiology. Renal physiology · 2015Article
- Shear stress-induced NO production is dependent on ATP autocrine signaling and capacitative calcium entry.Cellular and molecular bioengineering · 2014Article
- TRPV4 activation mediates flow-induced nitric oxide production in the rat thick ascending limb.American journal of physiology. Renal physiology · 2014Article
- Sustained resveratrol infusion increases natriuresis independent of renal vasodilation.Physiological reports · 2014Article
- Resveratrol increases nitric oxide production in the rat thick ascending limb via Ca2+/calmodulin.PloS one · 2014Article
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3 authors at 2 institutions in 1 country.
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Abstract
Mechanical stimulation caused by increasing flow induces nucleotide release from many cells. Luminal flow and extracellular ATP stimulate production of nitric oxide (NO) in thick ascending limbs. However, the factors that mediate flow-induced NO production are unknown. We hypothesized that luminal flow stimulates thick ascending limb NO production via ATP. We measured NO in isolated, perfused rat thick ascending limbs using the fluorescent dye DAF FM. The rate of increase in dye fluorescence reflects NO accumulation. Increasing luminal flow from 0 to 20 nl/min stimulated NO production from 17 ± 16 to 130 ± 37 arbitrary units (AU)/min (P < 0.02). Increasing flow from 0 to 20 nl/min raised ATP release from 4 ± 1 to 21 ± 6 AU/min (P < 0.04). Hexokinase (10 U/ml) plus glucose, which consumes ATP, completely prevented the measured increase in ATP. Luminal flow did not increase NO production in the presence of luminal and basolateral hexokinase (10 U/ml). When flow was increased with the ATPase apyrase in both luminal and basolateral solutions (5 U/ml), NO levels did not change significantly. The P2 receptor antagonist suramin (300 μmol/l) reduced flow-induced NO production by 83 ± 25% (P < 0.03) when added to both and basolateral sides. Luminal hexokinase decreased flow-induced NO production from 205.6 ± 85.6 to 36.6 ± 118.6 AU/min (P < 0.02). Basolateral hexokinase also reduced flow-induced NO production. The P2X receptor-selective antagonist NF023 (200 μmol/l) prevented flow-induced NO production when added to the basolateral side but not the luminal side. We conclude that ATP mediates flow-induced NO production in the thick ascending limb likely via activation of P2Y receptors in the luminal and P2X receptors in the basolateral membrane.
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