Evidence map›Paper›PMID 22495929›Full record

ArticleNucleic acids research2012

Negative regulation of miR-145 by C/EBP-β through the Akt pathway in cancer cells.

Mohit Sachdeva, Qian Liu, Julia Cao, Zhaohui Lu, Yin-Yuan Mo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed, 2 pooled it
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 2 syntheses or guidelines pooled it, 75 citations in OpenAlex.

  1. Pooled it
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  17. miR-145 Antagonizes SNAI1-Mediated Stemness and Radiation Resistance in Colorectal Cancer.Molecular therapy : the journal of the American Society of Gene Therapy · 2018
    Article
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Mohit SachdevaDepartment of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794, USA.
Qian Liu
Julia Cao
Zhaohui Lu
Yin-Yuan Mo
University of Mississippi Medical Center · USSouthern Illinois University School of Medicine · US

Funding

The Akt-associated microRNAs in cancer cellsR01CA154989 · NCI · UNIVERSITY OF MISSISSIPPI MED CTR · PI MO, YIN-YUAN · 2011 to 2015
$1.5M
NCI NIH HHS R01 CA154989
6 · The paper itself

Abstract

MicroRNAs are master gene regulators that can also be under the control of transcriptional regulation. We have previously shown that miR-145 is a tumor suppressor capable of silencing c-Myc and the tumor suppressor p53 induces miR-145 by directly binding to the miR-145 promoter, demonstrating the role of miR-145 in p53-mediated c-Myc repression. However, little is known as to why miR-145 is often downregulated in tumors. In this study, we identify CCAAT/enhancer binding protein beta (C/EBP-β) as a negative regulator for miR-145 expression by direct interaction with the putative C/EBP-β binding site in the miR-145 promoter. In the wild-type p53 background, C/EBP-β counteracts the ability of p53 to induce miR-145. Moreover, C/EBP-β is able to suppress miR-145 in the mutant p53 background, suggesting the p53 independent regulation of miR-145. Of interest, both the large isoform (LAP-2) and the small isoform (LIP) of C/EBP-β can exert suppressive function for miR-145. Finally, we further show that, like serum starvation and PI3K inhibitor LY29, the antioxidant resveratrol suppresses pAkt and phosphorylation of C/EBP-β and at the same time, it induces miR-145. Together, these results suggest a miR-145 regulatory system involving the Akt and C/EBP-β, which may contribute to the downregulation of miR-145 in cancer cells.

Indexed as

Gene Expression Regulation, NeoplasticAntineoplastic Agents, PhytogenicBinding SitesCCAAT-Enhancer-Binding Protein-betaCell Line, TumorFemaleHumansMicroRNAsPhosphorylationPromoter Regions, GeneticProto-Oncogene Proteins c-aktResveratrolSignal TransductionStilbenesTumor Suppressor Protein p53Antineoplastic Agents, PhytogenicCCAAT-Enhancer-Binding Protein-betaMicroRNAsProto-Oncogene Proteins c-aktResveratrolStilbenesTumor Suppressor Protein p53

Identifiers

PMID22495929
PMCPMC3413133
OpenAlexW2170326164

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.