ArticleThe Journal of general virology2012
Development of a reverse-genetics system for murine norovirus 3: long-term persistence occurs in the caecum and colon.
Article in The Journal of general virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 64 citations in OpenAlex.
- Egress-enhancing mutation reveals the inefficiency of non-enveloped virus cell exit.PLoS biology · 2025Article
- Plasmid-Based Reverse Genetics System Enabling One-Step Generation of Genotype 3 Hepatitis E Virus.Viruses · 2025Article
- Murine norovirus mutants adapted to replicate in human cells reveal a post-entry restriction.Journal of virology · 2024Article
- Intestinal tuft cell immune privilege enables norovirus persistence.Science immunology · 2024Article
- Murine norovirus mutants adapted to replicate in human cells reveal a post-entry restriction.bioRxiv : the preprint server for biology · 2024Article
- Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment.Journal of virology · 2023Article
- Infection of neonatal mice with the murine norovirus strain WU23 is a robust model to study norovirus pathogenesis.Lab animal · 2023Article
- Recent insights into reverse genetics of norovirus.Virus research · 2023Review
- Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity.Cell reports · 2022Article
- Murine norovirus virulence factor 1 (VF1) protein contributes to viral fitness during persistent infection.The Journal of general virology · 2021Article
- Review
- Article
- Article
- CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus.PLoS pathogens · 2020Article
- The intestinal regionalization of acute norovirus infection is regulated by the microbiota via bile acid-mediated priming of type III interferon.Nature microbiology · 2020Article
- Isolation and Identification of a Murine Norovirus Persistent Infection Strain in China.Frontiers in veterinary science · 2020Article
- Review
- Targeting macrophage- and intestinal epithelial cell-specific microRNAs against norovirus restricts replication in vivo.The Journal of general virology · 2018Article
- The Role of Interferon in Persistent Viral Infection: Insights from Murine Norovirus.Trends in microbiology · 2018Review
- Assessing the Efficacy of Small Molecule Inhibitors in aMouse Model of Persistent Norovirus Infection.Bio-protocol · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Human noroviruses (HuNoV) are a major cause of viral gastroenteritis worldwide, yet, due to the inability to propagate HuNoV in cell culture, murine norovirus (MNV) is typically used as a surrogate to study norovirus biology. MNV-3 represents an attractive strain to study norovirus infections in vivo because it establishes persistence in wild-type mice, yet causes symptoms resembling gastroenteritis in immune-compromised STAT1(-/-) mice. The lack of reverse-genetics approaches to recover genetically defined MNV-3 has limited further studies on the identification of viral sequences that contribute to persistence. Here we report the establishment of a combined DNA-based reverse-genetics and mouse-model system to study persistent MNV-3 infections in wild-type (C57BL/6) mice. Viral RNA and infectious virus were detected in faeces for at least 56 days after inoculation. Strikingly, the highest concentrations of viral RNA during persistence were detected in the caecum and colon, suggesting that viral persistence is maintained in these tissues. Possible adaptive changes arising during persistence in vivo appeared to accumulate in the minor capsid protein (VP2) and the viral polymerase (NS7), in contrast with adaptive mutations selected during cell-culture passages in RAW264.7 cells that appeared in the major capsid protein (VP1) and non-structural protein NS4. This system provides an attractive model that can be readily used to identify viral sequences that contribute to persistence in an immunocompetent host and to more acute infection in an immunocompromised host, providing new insights into the biology of norovirus infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.