ArticleThe Journal of biological chemistry2012
Plastocyanin controls the stabilization of the thylakoid Cu-transporting P-type ATPase PAA2/HMA8 in response to low copper in Arabidopsis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Article
- Acute copper stress showed toxic effects on the physiological metabolism of Ulva lactuca, a common green macroalgae.Scientific reports · 2024Article
- Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Plants (Basel, Switzerland) · 2024Article
- Article
- Copper accumulation in the aquatic fern Salvinia minima causes more severe physiological stress than zinc.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Article
- MtCOPT2 is a CuMycorrhiza · 2020Article
- SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana.Plant cell reports · 2019Article
- Conserved Cu-MicroRNAs inPlants (Basel, Switzerland) · 2019Article
- Article
- Heterologous Expression ofInternational journal of molecular sciences · 2018Article
- Comparative Transcriptome Profiles of Near-Isogenic Hexaploid Wheat Lines Differing for Effective Alleles at the 2DL FHB Resistance QTL.Frontiers in plant science · 2018Article
- Chloroplast Proteases: Updates on Proteolysis within and across Suborganellar Compartments.Plant physiology · 2016Review
- Combining -Omics to Unravel the Impact of Copper Nutrition on Alfalfa (Medicago sativa) Stem Metabolism.Plant & cell physiology · 2016Article
- Structural Insights into the Nucleotide-Binding Domains of the P1B-type ATPases HMA6 and HMA8 from Arabidopsis thaliana.PloS one · 2016Article
- Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics.Frontiers in plant science · 2016Review
- Comparative transcriptome analysis of grapevine in response to copper stress.Scientific reports · 2015Article
- HMA6 and HMA8 are two chloroplast Cu+-ATPases with different enzymatic properties.Bioscience reports · 2015Article
- Copper Delivery to Chloroplast Proteins and its Regulation.Frontiers in plant science · 2015Review
- Regulation of Cu delivery to chloroplast proteins.Plant signaling & behavior · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PAA2/HMA8 (P-type ATPase of Arabidopsis/Heavy-metal-associated 8) is a thylakoid located copper (Cu)-transporter in Arabidopsis thaliana. In tandem with PAA1/HMA6, which is located in the inner chloroplast envelope, it supplies Cu to plastocyanin (PC), an essential cuproenzyme of the photosynthetic machinery. We investigated whether the chloroplast Cu transporters are affected by Cu addition to the growth media. Immunoblots showed that PAA2 protein abundance decreased significantly and specifically when Cu in the media was increased, while PAA1 remained unaffected. The function of SPL7, the transcriptional regulator of Cu homeostasis, was not required for this regulation of PAA2 protein abundance and Cu addition did not affect PAA2 transcript levels, as determined by qRT-PCR. We used the translational inhibitor cycloheximide to analyze turnover and observed that the stability of the PAA2 protein was decreased in plants grown with elevated Cu. Interestingly, PAA2 protein abundance was significantly increased in paa1 mutants, in which the Cu content in the chloroplast is half of that of the wild-type, due to impaired Cu import into the organelle. In contrast in a pc2 insertion mutant, which has strongly reduced plastocyanin expression, the PAA2 protein levels were low regardless of Cu addition to the growth media. Together, these data indicate that plastid Cu levels control PAA2 stability and that plastocyanin, which is the target of PAA2 mediated Cu delivery in thylakoids, is a major determinant of this regulatory mechanism.
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