ArticleThe Journal of biological chemistry2012
Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 30 citations in OpenAlex.
- Metabolic engineering for microbial production of sugar acids.BMC biotechnology · 2025Review
- Identification of the enantiomeric nature of 2-keto-3-deoxy-galactonate in the catabolic pathway of 3,6-anhydro-L-galactose.Applied microbiology and biotechnology · 2023Article
- NADPJournal of microbiology and biotechnology · 2021Article
- Microbial hexuronate catabolism in biotechnology.AMB Express · 2019Review
- How Bioinformatic Tools Guide Experiments To Resolve the Chaos of Apparently Unlimited Metabolic Variation.Journal of bacteriology · 2019Article
- Novel Metabolic Pathways and Regulons for Hexuronate Utilization in Proteobacteria.Journal of bacteriology · 2019Article
- Ecological Conditions and Molecular Determinants Involved inFrontiers in plant science · 2019Review
- Three structurally and functionally distinct β-glucuronidases from the human gut microbeThe Journal of biological chemistry · 2018Article
- Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design.AMB Express · 2017Article
- Application of Nonphosphorylative Metabolism as an Alternative for Utilization of Lignocellulosic Biomass.Frontiers in microbiology · 2017Review
- A novel pathway for fungal D-glucuronate catabolism contains an L-idonate forming 2-keto-L-gulonate reductase.Scientific reports · 2016Article
- Engineering nonphosphorylative metabolism to generate lignocellulose-derived products.Nature chemical biology · 2016Article
- Involvement of Agrobacterium tumefaciens Galacturonate Tripartite ATP-Independent Periplasmic (TRAP) Transporter GaaPQM in Virulence Gene Expression.Applied and environmental microbiology · 2016Article
- Purification, crystallization and structural elucidation of D-galactaro-1,4-lactone cycloisomerase from Agrobacterium tumefaciens involved in pectin degradation.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Article
- Galactaro δ-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily.Biochemistry · 2014Article
- Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens.Acta crystallographica. Section F, Structural biology communications · 2014Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Microorganisms use different pathways for D-galacturonate catabolism. In the known microbial oxidative pathway, D-galacturonate is oxidized to D-galactarolactone, the lactone hydrolyzed to galactarate, which is further converted to 3-deoxy-2-keto-hexarate and α-ketoglutarate. We have shown recently that Agrobacterium tumefaciens strain C58 contains an uronate dehydrogenase (At Udh) that oxidizes D-galacturonic acid to D-galactarolactone. Here we report identification of a novel enzyme from the same A. tumefaciens strain, which we named Galactarolactone cycloisomerase (At Gci) (E.C. 5.5.1.-), for the direct conversion of the D-galactarolactone to 3-deoxy-2-keto-hexarate. The At Gci enzyme is 378 amino acids long and belongs to the mandelate racemase subgroup in the enolase superfamily. At Gci was heterologously expressed in Escherichia coli, and the purified enzyme was found to exist as an octameric form. It is active both on D-galactarolactone and D-glucarolactone, but does not work on the corresponding linear hexaric acid forms. The details of the reaction mechanism were further studied by NMR and optical rotation demonstrating that the reaction product of At Gci from D-galactaro-1,4-lactone and D-glucaro-1,4-lactone conversion is in both cases the L-threo form of 3-deoxy-2-keto-hexarate.
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