ArticleJournal of clinical microbiology2012
Use of urine with nested PCR targeting the flagellin gene (fliC) for diagnosis of typhoid fever.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Meta-Analysis of Typhoid Diagnostic Accuracy Studies: A Recommendation to Adopt a Standardized Composite Reference.PloS one · 2015Pooled it
- Evaluation of mdh, dld, tcfA, and folE gene markers for detection of enteric fever using real-time PCR.Scientific reports · 2026Article
- FromACS omega · 2023Review
- Clinical Evaluation of a Multiplex PCR for the Detection ofThe American journal of tropical medicine and hygiene · 2019Article
- Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae.Clinical microbiology reviews · 2019Review
- Organoid and Enteroid Modeling ofFrontiers in cellular and infection microbiology · 2018Review
- Identification of Five NovelBioMed research international · 2016Article
- Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections.Clinical microbiology reviews · 2015Review
- Diagnostics for invasive Salmonella infections: Current challenges and future directions.Vaccine · 2015Review
- Mix-infection of S. Typhi and ParaTyphi A in Typhoid Fever and Chronic Typhoid Carriers: A Nested PCR Based Study in North India.Journal of clinical and diagnostic research : JCDR · 2014Article
- Salmonella chronic carriage: epidemiology, diagnosis, and gallbladder persistence.Trends in microbiology · 2014Review
- Cellular and cytokine responses to Salmonella enterica serotype Typhi proteins in patients with typhoid fever in Bangladesh.The American journal of tropical medicine and hygiene · 2014Article
- Evaluation of a typhoid/paratyphoid diagnostic assay (TPTest) detecting anti-Salmonella IgA in secretions of peripheral blood lymphocytes in patients in Dhaka, Bangladesh.PLoS neglected tropical diseases · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was planned to evaluate the efficacy of the use of nested PCR with a large volume of easily available urine as an effort to devise a test that can meet the levels necessary to be considered a gold standard for the diagnosis of typhoid fever. A total of 60 subjects with suspected cases of typhoid fever and 20 apparently healthy control subjects were included in the study. The study period extended from March 2010 to June 2011. Blood, urine, and stool specimens were collected from the participating individuals. Nested PCR was done targeting the flagellin gene (fliC) of Salmonella enterica subspecies enterica serotype Typhi. Specimens in all three categories could be collected from 22 of the subjects with suspected cases of typhoid fever; 21 of the 22 urine samples (95.4%) yielded a desired amplicon of 343 bp, whereas none of the urine samples collected from the 20 control subjects (0%) yielded the amplicon. The analyses of blood and stool samples were found to be inferior to urine sample analysis in sensitivity, with detection rates of 90.9% and 68.1%, respectively. Culture isolation was observed to display very poor sensitivity (31.8%). A large volume of urine may be the ideal specimen for PCR-based detection of typhoid fever.
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