ArticleThe Journal of biological chemistry2012
Low NO concentration dependence of reductive nitrosylation reaction of hemoglobin.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Acute therapy with hemopexin (CSL889) resolves LPS-induced lung vaso-occlusion in sickle mice.Blood vessels, thrombosis & hemostasis · 2026Article
- Immobilized neuroglobin scavenges carbon monoxide from circulating carboxyhemoglobin.bioRxiv : the preprint server for biology · 2026Article
- Cytochrome bFree radical biology & medicine · 2026Article
- Comparative Transcriptomics Reveals Metabolic Adaptations ofMicroorganisms · 2026Article
- Tumor Microenvironment-responsive Nanocatalyst for Targeted Chemodynamic Cancer Therapy.Advanced healthcare materials · 2025Article
- The chemical biology of dinitrogen trioxide.Redox biochemistry and chemistry · 2024Article
- Mechanistic insights into cell-free hemoglobin-induced injury during septic shock.American journal of physiology. Heart and circulatory physiology · 2021Article
- Myoglobin promotes nitrite-dependent mitochondrial S-nitrosation to mediate cytoprotection after hypoxia/reoxygenation.Nitric oxide : biology and chemistry · 2020Article
- Tandem P-selectin glycoprotein ligand immunoglobulin prevents lung vaso-occlusion in sickle cell disease mice.Experimental hematology · 2020Article
- Regulation of erythrocyte NaThe Journal of biological chemistry · 2019Article
- Hemoglobin inhibits albumin uptake by proximal tubule cells: implications for sickle cell disease.American journal of physiology. Cell physiology · 2017Article
- HbE/β-Thalassemia and Oxidative Stress: The Key to Pathophysiological Mechanisms and Novel Therapeutics.Antioxidants & redox signaling · 2017Review
- A mathematical model for the role of NNitric oxide : biology and chemistry · 2016Article
- Reactions between nitrosopersulfide and heme proteins.Free radical biology & medicine · 2016Article
- Can erythrocytes release biologically active NO?Cell communication and signaling : CCS · 2016Article
- Erythrocytes do not activate purified and platelet soluble guanylate cyclases even in conditions favourable for NO synthesis.Cell communication and signaling : CCS · 2016Article
- Convergence of biological nitration and nitrosation via symmetrical nitrous anhydride.Nature chemical biology · 2015Article
- Pitfalls in measuring NO bioavailability using NOx.Nitric oxide : biology and chemistry · 2015Article
- Evaluating the capacity to generate and preserve nitric oxide bioactivity in highly purified earthworm erythrocruorin: a giant polymeric hemoglobin with potential blood substitute properties.The Journal of biological chemistry · 2015Article
- The globin superfamily: functions in nitric oxide formation and decay.Biological chemistry · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The reductive nitrosylation of ferric (met)hemoglobin is of considerable interest and remains incompletely explained. We have previously observed that at low NO concentrations the reaction with tetrameric hemoglobin occurs with an observed rate constant that is at least 5 times faster than that observed at higher concentrations. This was ascribed to a faster reaction of NO with a methemoglobin-nitrite complex. We now report detailed studies of this reaction of low NO with methemoglobin. Nitric oxide paradoxically reacts with ferric hemoglobin with faster observed rate constants at the lower NO concentration in a manner that is not affected by changes in nitrite concentration, suggesting that it is not a competition between NO and nitrite, as we previously hypothesized. By evaluation of the fast reaction in the presence of allosteric effectors and isolated β- and α-chains of hemoglobin, it appears that NO reacts with a subpopulation of β-subunit ferric hemes whose population is influenced by quaternary state, redox potential, and hemoglobin dimerization. To further characterize the role of nitrite, we developed a system that oxidizes nitrite to nitrate to eliminate nitrite contamination. Removal of nitrite does not alter reaction kinetics, but modulates reaction products, with a decrease in the formation of S-nitrosothiols. These results are consistent with the formation of NO(2)/N(2)O(3) in the presence of nitrite. The observed fast reductive nitrosylation observed at low NO concentrations may function to preserve NO bioactivity via primary oxidation of NO to form nitrite or in the presence of nitrite to form N(2)O(3) and S-nitrosothiols.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.