ArticleProceedings of the National Academy of Sciences of the United States of America2012
Using translational enhancers to increase transgene expression in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 239 papers.
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Who cites it
239 citing papers in PubMed, 449 citations in OpenAlex.
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- Differential neuronal survival defines a novel axis of sexual dimorphism in the Drosophila brain.Cell genomics · 2026Article
- Neural circuits regulating social dominance implement a strategy predicted by evolutionary game theory.bioRxiv : the preprint server for biology · 2026Article
- Optogenetic control of transition to metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Feeding decision-making by a single neuron via disparate neurotransmitters.Nature communications · 2026Article
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- Drosophila DNp03 descending neurons serve as a hub within a flight saccade network.Current biology : CB · 2026Article
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- HIF-1α-mediated feedback prevents TOR signalling from depleting oxygen supply and triggering stress during normal development.Nature communications · 2025Article
179 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to specify the expression levels of exogenous genes inserted in the genomes of transgenic animals is critical for the success of a wide variety of experimental manipulations. Protein production can be regulated at the level of transcription, mRNA transport, mRNA half-life, or translation efficiency. In this report, we show that several well-characterized sequence elements derived from plant and insect viruses are able to function in Drosophila to increase the apparent translational efficiency of mRNAs by as much as 20-fold. These increases render expression levels sufficient for genetic constructs previously requiring multiple copies to be effective in single copy, including constructs expressing the temperature-sensitive inactivator of neuronal function Shibire(ts1), and for the use of cytoplasmic GFP to image the fine processes of neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.