ArticleProceedings of the National Academy of Sciences of the United States of America2012
Cryo-EM structure of a transcribing cypovirus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Insect cypoviruses: advances in structural characterization, evolutionary genomics, and host interaction dynamics.Archives of virology · 2026Review
- Review
- Cryo-EM structures of Banna virus in multiple states reveal stepwise detachment of viral spikes.Nature communications · 2024Article
- The structure of a 12-segmented dsRNA reovirus: New insights into capsid stabilization and organization.PLoS pathogens · 2023Article
- Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture.PLoS pathogens · 2023Article
- In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structures of L-BC virus and its open particle provide insight into Totivirus capsid assembly.Communications biology · 2022Article
- Crystal Structures of Flavivirus NS5 Guanylyltransferase Reveal a GMP-Arginine Adduct.Journal of virology · 2022Article
- Multiple conformations of trimeric spikes visualized on a non-enveloped virus.Nature communications · 2022Article
- Viral Capsid and Polymerase in Reoviridae.Sub-cellular biochemistry · 2022Article
- Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission.PLoS pathogens · 2021Article
- Simulation-Based Methods for Model Building and Refinement in Cryoelectron Microscopy.Journal of chemical information and modeling · 2020Review
- Hands on Methods for High Resolution Cryo-Electron Microscopy Structures of Heterogeneous Macromolecular Complexes.Frontiers in molecular biosciences · 2019Review
- Structure of RNA polymerase complex and genome within a dsRNA virus provides insights into the mechanisms of transcription and assembly.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Exogenous gene can be expressed by a recombinant Bombyx mori cypovirus.Applied microbiology and biotechnology · 2018Article
- Cypovirus capsid protein VP5 has nucleoside triphosphatase activity.Virologica Sinica · 2017Article
- Biological cryo-electron microscopy in China.Protein science : a publication of the Protein Society · 2017Review
- De Novo modeling in cryo-EM density maps with Pathwalking.Journal of structural biology · 2016Article
- Identification and characterization of two cleavage fragments from the Aquareovirus nonstructural protein NS80.Virologica Sinica · 2016Article
- Exploring the inside details of virions by electron microscopy.Biophysics reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double-stranded RNA viruses in the family Reoviridae are capable of transcribing and capping nascent mRNA within an icosahedral viral capsid that remains intact throughout repeated transcription cycles. However, how the highly coordinated mRNA transcription and capping process is facilitated by viral capsid proteins is still unknown. Cypovirus provides a good model system for studying the mRNA transcription and capping mechanism of viruses in the family Reoviridae. Here, we report a full backbone model of a transcribing cypovirus built from a near-atomic-resolution density map by cryoelectron microscopy. Compared with the structure of a nontranscribing cypovirus, the major capsid proteins of transcribing cypovirus undergo a series of conformational changes, giving rise to structural changes in the capsid shell: (i) an enlarged capsid chamber, which provides genomic RNA with more flexibility to move within the densely packed capsid, and (ii) a widened peripentonal channel in the capsid shell, which we confirmed to be a pathway for nascent mRNA. A rod-like structure attributable to a partially resolved nascent mRNA was observed in this channel. In addition, conformational change in the turret protein results in a relatively open turret at each fivefold axis. A GMP moiety, which is transferred to 5'-diphosphorylated mRNA during the mRNA capping reaction, was identified in the pocket-like guanylyltransferase domain of the turret protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.