ArticleBiology of reproduction2012
Complex N-glycans are essential, but core 1 and 2 mucin O-glycans, O-fucose glycans, and NOTCH1 are dispensable, for mammalian spermatogenesis.
Article in Biology of reproduction, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Spatial Organization of the Sperm Cell Glycoproteome.Molecular & cellular proteomics : MCP · 2025Article
- Inferring post-transcriptional regulation within and across cell types in human testis.bioRxiv : the preprint server for biology · 2024Article
- Genetics of glycosylation in mammalian development and disease.Nature reviews. Genetics · 2024Review
- Unlocking Genetic Mysteries during the Epic Sperm Journey toward Fertilization: Further ExpandingBiomolecules · 2024Review
- Deletion ofFrontiers in cell and developmental biology · 2024Article
- Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.Journal of cell communication and signaling · 2022Article
- MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice.International journal of molecular sciences · 2021Article
- C1GALT1 in health and disease.Oncology letters · 2021Review
- The Role of Extracellular Vesicles in Sperm Function and Male Fertility.Sub-cellular biochemistry · 2021Review
- Point mutations that inactivate MGAT4D-L, an inhibitor of MGAT1 and complexThe Journal of biological chemistry · 2020Article
- RNA-sequencing and bioinformatics analysis of long noncoding RNAs and mRNAs in the asthenozoospermia.Bioscience reports · 2020Observational
- Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.Cell proliferation · 2020Article
- 3D Structure and Function of Glycosyltransferases Involved inInternational journal of molecular sciences · 2020Review
- The Intergenerational Impacts of Paternal Diet on DNA Methylation and Offspring Phenotypes in Sheep.Frontiers in genetics · 2020Article
- Transgenic Rescue of Spermatogenesis in Males WithFrontiers in cell and developmental biology · 2020Article
- Roles for Golgi Glycans in Oogenesis and Spermatogenesis.Frontiers in cell and developmental biology · 2019Review
- β1,4-Galactosyltransferase V activates Notch1 signaling in glioma stem-like cells and promotes their transdifferentiation into endothelial cells.The Journal of biological chemistry · 2018Article
- MGAT1 and Complex N-Glycans Regulate ERK Signaling During Spermatogenesis.Scientific reports · 2018Article
- Formation of multiple-oocyte follicles in culture.In vitro cellular & developmental biology. Animal · 2017Article
- What Have We Learned from Glycosyltransferase Knockouts in Mice?Journal of molecular biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
To identify roles in spermatogenesis for major subclasses of N- and O-glycans and Notch signaling, male mice carrying floxed C1galt1, Pofut1, Notch1 or Mgat1 alleles and a testis-specific Cre recombinase transgene were generated. T-synthase (C1GALT1) transfers Gal to generate core 1 and core 2 mucin O-glycans; POFUT1 transfers O-fucose to particular epidermal growth factor-like repeats and is essential for canonical Notch signaling; and MGAT1 (GlcNAcT-I) transfers GlcNAc to initiate hybrid and complex N-glycan synthesis. Cre recombinase transgenes driven by various promoters were investigated, including Stra8-iCre expressed in spermatogonia, Sycp1-Cre expressed in spermatocytes, Prm1-Cre expressed in spermatids, and AMH-Cre expressed in Sertoli cells. All Cre transgenes deleted floxed alleles, but efficiencies varied widely. Stra8-iCre was the most effective, deleting floxed Notch1 and Mgat1 alleles with 100% efficiency and floxed C1galt1 and Pofut1 alleles with ~80% efficiency, based on transmission of deleted alleles. Removal of C1galt1, Pofut1, or Notch1 in spermatogonia had no effect on testicular weight, histology, or fertility. However, males in which the synthesis of complex N-glycans was blocked by deletion of Mgat1 in spermatogonia did not produce sperm. Spermatogonia, spermatocytes, and spermatids were generated, but most spermatids formed giant multinucleated cells or symplasts, and apoptosis was increased. Therefore, although core 1 and 2 mucin O-glycans, NOTCH1, POFUT1, O-fucose glycans, and Notch signaling are dispensable, MGAT1 and complex N-glycans are essential for spermatogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.