ArticleNucleic acids research2012
A rapid method for assessing the RNA-binding potential of a protein.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- In vivo regulation of bacterial Rho-dependent transcription termination by the nascent RNA.The Journal of biological chemistry · 2022Article
- A Novel Family of RNA-Binding Proteins Regulate Polysaccharide Metabolism inJournal of bacteriology · 2021Article
- Article
- A crucial RNA-binding lysine residue in the Nab3 RRM domain undergoes SET1 and SET3-responsive methylation.Nucleic acids research · 2020Article
- The archaeal RNA chaperone TRAM0076 shapes the transcriptome and optimizes the growth of Methanococcus maripaludis.PLoS genetics · 2019Article
- Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription.Scientific reports · 2018Article
- A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B.PLoS genetics · 2017Article
- Conserved TRAM Domain Functions as an Archaeal Cold Shock Protein via RNA Chaperone Activity.Frontiers in microbiology · 2017Article
- p73 expression is regulated by ribosomal protein RPL26 through mRNA translation and protein stability.Oncotarget · 2016Article
- The zinc fingers of YY1 bind single-stranded RNA with low sequence specificity.Nucleic acids research · 2016Article
- The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.Protein science : a publication of the Protein Society · 2016Article
- Systematic protein interactome analysis of glycosaminoglycans revealed YcbS as a novel bacterial virulence factor.Scientific reports · 2016Article
- Novel RNA-binding protein P311 binds eukaryotic translation initiation factor 3 subunit b (eIF3b) to promote translation of transforming growth factor β1-3 (TGF-β1-3).The Journal of biological chemistry · 2014Article
- RNA-dependent chromatin localization of KDM4D lysine demethylase promotes H3K9me3 demethylation.Nucleic acids research · 2014Article
- Noncoding RNAs and LRRFIP1 regulate TNF expression.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Light activation of Staphylococcus aureus toxin YoeBSa1 reveals guanosine-specific endoribonuclease activity.Biochemistry · 2014Article
- Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry.RNA (New York, N.Y.) · 2012Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, evidence has emerged for the existence of many diverse types of RNA, which play roles in a wide range of biological processes in all kingdoms of life. These molecules generally do not, however, act in isolation, and identifying which proteins partner with RNA is a major challenge. Many methods, in vivo and in vitro, have been used to address this question, including combinatorial or high-throughput approaches, such as systematic evolution of ligands, cross-linking and immunoprecipitation and RNA immunoprecipitation combined with deep sequencing. However, most of these methods are not trivial to pursue and often require substantial optimization before results can be achieved. Here, we demonstrate a simple technique that allows one to screen proteins for RNA-binding properties in a gel-shift experiment and can be easily implemented in any laboratory. This assay should be a useful first-pass tool for assessing whether a protein has RNA- or DNA-binding properties, prior to committing resources to more complex procedures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.