Evidence map›Paper›PMID 22492441›Full record

ArticleApplied and environmental microbiology2012

Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.

Erica M Hartmann, Jonathan P Badalamenti, Rosa Krajmalnik-Brown, Rolf U Halden

Abstract readEvaluation Study
In one paragraph

Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. The Divided Bacterial Genome: Structure, Function, and Evolution.Microbiology and molecular biology reviews : MMBR · 2017
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Erica M HartmannThe Swette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
Jonathan P Badalamenti
Rosa Krajmalnik-Brown
Rolf U Halden

Funding

Novel Approaches to Studying the Situ Bioremediation of Complex MixturesR01ES015445 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI HALDEN, ROLF U · 2006 to 2009
$1.0M
NIEHS NIH HHS R01 ES015445NIEHS NIH HHS R01ES015445
6 · The paper itself

Abstract

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.

Indexed as

BiotransformationPlasmidsSoil MicrobiologyBacteriological TechniquesDioxinsGenes, BacterialMetabolic Networks and PathwaysReal-Time Polymerase Chain ReactionSphingomonasdibenzo(1,4)dioxinDioxins

Identifiers

PMID22492441
PMCPMC3370553

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.