ArticleDevelopment (Cambridge, England)2012
Dynamic in vivo binding of transcription factors to cis-regulatory modules of cer and gsc in the stepwise formation of the Spemann-Mangold organizer.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Combinatorial regulatory states define cell fate diversity during embryogenesis.Nature communications · 2024Article
- Recognition of H2AK119ub plays an important role in RSF1-regulated earlyFrontiers in cell and developmental biology · 2023Article
- Uncovering the mesendoderm gene regulatory network through multi-omic data integration.Cell reports · 2022Article
- The Organizer and Its Signaling in Embryonic Development.Journal of developmental biology · 2021Review
- Temporal transcriptomic profiling reveals dynamic changes in gene expression of Xenopus animal cap upon activin treatment.Scientific reports · 2021Article
- Segregation of brain and organizer precursors is differentially regulated by Nodal signaling at blastula stage.Biology open · 2021Article
- Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation.Cell reports · 2019Article
- Evolution of cis-regulatory modules for the head organizer geneZoological letters · 2019Article
- The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development.Scientific reports · 2018Article
- TGF-β Family Signaling in Early Vertebrate Development.Cold Spring Harbor perspectives in biology · 2018Review
- A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs.Seminars in cell & developmental biology · 2017Review
- Vertebrate Axial Patterning: From Egg to Asymmetry.Advances in experimental medicine and biology · 2017Review
- Neural transcription factors bias cleavage stage blastomeres to give rise to neural ectoderm.Genesis (New York, N.Y. : 2000) · 2016Article
- Transcription factors Mix1 and VegT, relocalization of vegt mRNA, and conserved endoderm and dorsal specification in frogs.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Molecular specification of germ layers in vertebrate embryos.Cellular and molecular life sciences : CMLS · 2016Review
- Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor β (TGFβ)-mediated Germ Layer Induction in Xenopus Embryos.The Journal of biological chemistry · 2015Article
- Transcription factor p63 bookmarks and regulates dynamic enhancers during epidermal differentiation.EMBO reports · 2015Article
- Early neural ectodermal genes are activated by Siamois and Twin during blastula stages.Genesis (New York, N.Y. : 2000) · 2015Article
- Occupancy of tissue-specific cis-regulatory modules by Otx2 and TLE/Groucho for embryonic head specification.Nature communications · 2014Article
- Self-regulation of the head-inducing properties of the Spemann organizer.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How multiple developmental cues are integrated on cis-regulatory modules (CRMs) for cell fate decisions remains uncertain. The Spemann-Mangold organizer in Xenopus embryos expresses the transcription factors Lim1/Lhx1, Otx2, Mix1, Siamois (Sia) and VegT. Reporter analyses using sperm nuclear transplantation and DNA injection showed that cerberus (cer) and goosecoid (gsc) are activated by the aforementioned transcription factors through CRMs conserved between X. laevis and X. tropicalis. ChIP-qPCR analysis for the five transcription factors revealed that cer and gsc CRMs are initially bound by both Sia and VegT at the late blastula stage, and subsequently bound by all five factors at the gastrula stage. At the neurula stage, only binding of Lim1 and Otx2 to the gsc CRM, among others, persists, which corresponds to their co-expression in the prechordal plate. Based on these data, together with detailed expression pattern analysis, we propose a new model of stepwise formation of the organizer, in which (1) maternal VegT and Wnt-induced Sia first bind to CRMs at the blastula stage; then (2) Nodal-inducible Lim1, Otx2, Mix1 and zygotic VegT are bound to CRMs in the dorsal endodermal and mesodermal regions where all these genes are co-expressed; and (3) these two regions are combined at the gastrula stage to form the organizer. Thus, the in vivo dynamics of multiple transcription factors highlight their roles in the initiation and maintenance of gene expression, and also reveal the stepwise integration of maternal, Nodal and Wnt signaling on CRMs of organizer genes to generate the organizer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.