ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2012
"Silent" dissemination of Klebsiella pneumoniae isolates bearing K. pneumoniae carbapenemase in a long-term care facility for children and young adults in Northeast Ohio.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
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- Klebsiella pneumoniae employs a type VI secretion system to overcome microbiota-mediated colonization resistance.Nature communications · 2025Article
- Benefits and Safety of Empiric Antibiotic Treatment Active Against KPC-ProducingAntibiotics (Basel, Switzerland) · 2024Article
- Deciphering the gastrointestinal carriage ofInfection and immunity · 2024Review
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- Molecular Characterization and Epidemiology of Carbapenem-ResistantThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Article
- A Novel Peptide as a Specific and Selective Probe forBiosensors · 2022Article
- The Epidemiology, Virulence and Antimicrobial Resistance of InvasiveInfection and drug resistance · 2021Article
- Animal Model To Study Klebsiella pneumoniae Gastrointestinal Colonization and Host-to-Host Transmission.Infection and immunity · 2020Article
- Notes from the Field: Candida auris and Carbapenemase-Producing Organism Prevalence in a Pediatric Hospital Providing Long-Term Transitional Care - Chicago, Illinois, 2019.MMWR. Morbidity and mortality weekly report · 2020Article
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- ChromIDFrontiers in microbiology · 2020Article
- A Multi-Centered Case-Case-Control Study of Factors Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacteriaceae Infections in Children and Young Adults.The Pediatric infectious disease journal · 2019Article
- Future Challenges in Pediatric and Neonatal Sepsis: Emerging Pathogens and Antimicrobial Resistance.Journal of pediatric intensive care · 2019Review
- Increased Mortality Among Carbapenemase-Producing Carbapenem-ResistantOpen forum infectious diseases · 2019Article
- Antimicrobial stewardship in pediatric post-acute care facilities.American journal of infection control · 2018Article
- Colistin Versus Ceftazidime-Avibactam in the Treatment of Infections Due to Carbapenem-Resistant Enterobacteriaceae.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Observational
- Colonization, Infection, and the Accessory Genome ofFrontiers in cellular and infection microbiology · 2018Review
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11 authors.
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Abstract
backgroundKlebsiella pneumoniae isolates harboring the K. pneumoniae carbapenemase gene (bla(KPC)) are creating a significant healthcare threat in both acute and long-term care facilities (LTCFs). As part of a study conducted in 2004 to determine the risk of stool colonization with extended-spectrum cephalosporin-resistant gram-negative bacteria, 12 isolates of K. pneumoniae that exhibited nonsusceptibility to extended-spectrum cephalosporins were detected. All were gastrointestinal carriage isolates that were not associated with infection.
methodsReassessment of the carbapenem minimum inhibitory concentrations using revised 2011 Clinical Laboratory Standards Institute breakpoints uncovered carbapenem resistance. To further investigate, a DNA microarray assay, PCR-sequencing of bla genes, immunoblotting, repetitive-sequence-based PCR (rep-PCR) and multilocus sequence typing (MLST) were performed.
resultsThe DNA microarray detected bla(KPC) in all 12 isolates, and bla(KPC-3) was identified by PCR amplification and sequencing of the amplicon. In addition, a bla(SHV-11) gene was detected in all isolates. Immunoblotting revealed "low-level" production of the K. pneumoniae carbapenemase, and rep-PCR indicated that all bla(KPC-3)-positive K. pneumoniae strains were genetically related (≥98% similar). According to MLST, all isolates belonged to sequence type 36. This sequence type has not been previously linked with bla(KPC) carriage. Plasmids from 3 representative isolates readily transferred the bla(KPC-3) to Escherichia coli J-53 recipients.
conclusionsOur findings reveal the "silent" dissemination of bla(KPC-3) as part of Tn4401b on a mobile plasmid in Northeast Ohio nearly a decade ago and establish the first report, to our knowledge, of K. pneumoniae containing bla(KPC-3) in an LTCF caring for neurologically impaired children and young adults.
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