ArticleMolecular therapy : the journal of the American Society of Gene Therapy2012
SCL/TAL1 regulates hematopoietic specification from human embryonic stem cells.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 66 citations in OpenAlex.
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- The genesis of human hematopoietic stem cells.Blood · 2023Article
- The aberrant epigenome ofGenome research · 2023Article
- De Novo Generation of Human Hematopoietic Stem Cells from Pluripotent Stem Cells for Cellular Therapy.Cells · 2023Review
- A Benchmark Side-by-Side Comparison of Two Well-Established Protocols forFrontiers in cell and developmental biology · 2021Article
- MSX2 suppression through inhibition of TGFβ signaling enhances hematopoietic differentiation of human embryonic stem cells.Stem cell research & therapy · 2020Article
- Article
- Hematopoietic Differentiation of Human Pluripotent Stem Cells: HOX and GATA Transcription Factors as Master Regulators.Current genomics · 2019Review
- Gene therapy of hematological disorders: current challenges.Gene therapy · 2019Review
- Understanding the Journey of Human Hematopoietic Stem Cell Development.Stem cells international · 2019Review
- MEIS2 regulates endothelial to hematopoietic transition of human embryonic stem cells by targeting TAL1.Stem cell research & therapy · 2018Article
- Early Human Hemogenic Endothelium Generates Primitive and Definitive Hematopoiesis In Vitro.Stem cell reports · 2018Article
- Identification and Phylogenetic Analysis of Basic Helix-Loop-Helix Genes in the Diamondback Moth.Journal of insect science (Online) · 2018Article
- MEIS1 Regulates Hemogenic Endothelial Generation, Megakaryopoiesis, and Thrombopoiesis in Human Pluripotent Stem Cells by Targeting TAL1 and FLI1.Stem cell reports · 2018Article
- Downregulation of LGR5 Expression Inhibits Cardiomyocyte Differentiation and Potentiates Endothelial Differentiation from Human Pluripotent Stem Cells.Stem cell reports · 2017Article
- Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2017Article
- Intragenic CpG islands play important roles in bivalent chromatin assembly of developmental genes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Development Refractoriness of MLL-Rearranged Human B Cell Acute Leukemias to Reprogramming into Pluripotency.Stem cell reports · 2016Article
- A synthetic three-dimensional niche system facilitates generation of functional hematopoietic cells from human-induced pluripotent stem cells.Journal of hematology & oncology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Determining the molecular regulators/pathways responsible for the specification of human embryonic stem cells (hESCs) into hematopoietic precursors has far-reaching implications for potential cell therapies and disease modeling. Mouse models lacking SCL/TAL1 (stem cell leukemia/T-cell acute lymphocytic leukemia 1) do not survive beyond early embryogenesis because of complete absence of hematopoiesis, indicating that SCL is a master early hematopoietic regulator. SCL is commonly found rearranged in human leukemias. However, there is barely information on the role of SCL on human embryonic hematopoietic development. Differentiation and sorting assays show that endogenous SCL expression parallels hematopoietic specification of hESCs and that SCL is specifically expressed in hematoendothelial progenitors (CD45(-)CD31(+)CD34(+)) and, to a lesser extent, on CD45(+) hematopoietic cells. Enforced expression of SCL in hESCs accelerates the emergence of hematoendothelial progenitors and robustly promotes subsequent differentiation into primitive (CD34(+)CD45(+)) and total (CD45(+)) blood cells with higher clonogenic potential. Short-hairpin RNA-based silencing of endogenous SCL abrogates hematopoietic specification of hESCs, confirming the early hematopoiesis-promoting effect of SCL. Unfortunately, SCL expression on its own is not sufficient to confer in vivo engraftment to hESC-derived hematopoietic cells, suggesting that additional yet undefined master regulators are required to orchestrate the stepwise hematopoietic developmental process leading to the generation of definitive in vivo functional hematopoiesis from hESCs.
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