ArticleHeredity2012
Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus.
Article in Heredity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 75 citations in OpenAlex.
- Evolutionary dynamics under phenotypic uncertainty.bioRxiv : the preprint server for biology · 2026Article
- The importance of epistasis in the evolution of viral pathogens.Virus evolution · 2025Review
- Mechanistic causes of sign epistasis and its applications.Frontiers in genetics · 2024Review
- Article
- Mutational robustness changes during long-term adaptation in laboratory budding yeast populations.eLife · 2022Article
- Article
- Patterns and Causes of Signed Linkage Disequilibria in Flies and Plants.Molecular biology and evolution · 2021Article
- ΦX174 Attenuation by Whole-Genome Codon Deoptimization.Genome biology and evolution · 2021Article
- Increasing temperature alters the within-host competition of viral strains and influences virus genetic variability.Virus evolution · 2021Article
- Direct coupling analysis of epistasis in allosteric materials.PLoS computational biology · 2020Article
- Recent insights into the genotype-phenotype relationship from massively parallel genetic assays.Evolutionary applications · 2019Review
- An experimental assay of the interactions of amino acids from orthologous sequences shaping a complex fitness landscape.PLoS genetics · 2019Article
- Mutagenesis Scanning Uncovers Evolutionary Constraints on Tobacco Etch Potyvirus Membrane-Associated 6K2 Protein.Genome biology and evolution · 2019Article
- Existing Host Range Mutations Constrain Further Emergence of RNA Viruses.Journal of virology · 2019Article
- Analysis of Fitness Trade-Offs in the Host Range Expansion of an RNA Virus, Tobacco Mild Green Mosaic Virus.Journal of virology · 2018Article
- Unraveling the causes of adaptive benefits of synonymous mutations in TEM-1 β-lactamase.Heredity · 2018Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- Strain-dependent mutational effects for Pepino mosaic virus in a natural host.BMC evolutionary biology · 2017Article
- Efficient escape from local optima in a highly rugged fitness landscape by evolving RNA virus populations.Proceedings. Biological sciences · 2016Article
- RNA Silencing May Play a Role in but Is Not the Only Determinant of the Multiplicity of Infection.Journal of virology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How epistatic interactions between mutations determine the genetic architecture of fitness is of central importance in evolution. The study of epistasis is particularly interesting for RNA viruses because of their genomic compactness, lack of genetic redundancy, and apparent low complexity. Moreover, interactions between mutations in viral genomes determine traits such as resistance to antiviral drugs, virulence and host range. In this study we generated 53 Tobacco etch potyvirus genotypes carrying pairs of single-nucleotide substitutions and measured their separated and combined deleterious fitness effects. We found that up to 38% of pairs had significant epistasis for fitness, including both positive and negative deviations from the null hypothesis of multiplicative effects. Interestingly, the sign of epistasis was correlated with viral protein-protein interactions in a model network, being predominantly positive between linked pairs of proteins and negative between unlinked ones. Furthermore, 55% of significant interactions were cases of reciprocal sign epistasis (RSE), indicating that adaptive landscapes for RNA viruses maybe highly rugged. Finally, we found that the magnitude of epistasis correlated negatively with the average effect of mutations. Overall, our results are in good agreement to those previously reported for other viruses and further consolidate the view that positive epistasis is the norm for small and compact genomes that lack genetic robustness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.