ArticleBiological procedures online2012
A reinvestigation of somatic hypermethylation at the PTEN CpG island in cancer cell lines.
Article in Biological procedures online, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Review
- Toward Systems Pathology for PTEN Diagnostics.Cold Spring Harbor perspectives in medicine · 2020Review
- Exosomal miR-21 regulates the TETs/PTENp1/PTEN pathway to promote hepatocellular carcinoma growth.Molecular cancer · 2019Article
- Revisiting the Clinical and Biologic Relevance of Partial PTEN Loss in Melanoma.The Journal of investigative dermatology · 2019Article
- PTENP1 inhibits the growth of esophageal squamous cell carcinoma by regulating SOCS6 expression and correlates with disease prognosis.Molecular carcinogenesis · 2017Article
- [Role of lncRNA PTENP1 in tumorigenesis and progression of bladder cancer and the molecular mechanism].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Decreased expression of pseudogene PTENP1 promotes malignant behaviours and is associated with the poor survival of patients with HNSCC.Scientific reports · 2017Article
- DNA methylation of the LIN28 pseudogene family.BMC genomics · 2015Article
- Current clinical regulation of PI3K/PTEN/Akt/mTOR signalling in treatment of human cancer.Journal of cancer research and clinical oncology · 2015Review
- Focus on PTEN Regulation.Frontiers in oncology · 2015Review
- Regulation of mammary epithelial cell homeostasis by lncRNAs.The international journal of biochemistry & cell biology · 2014Review
- The importance of distinguishing pseudogenes from parental genes.Clinical epigenetics · 2014Article
- Impact of decitabine on immunohistochemistry expression of the putative tumor suppressor genes FHIT, WWOX, FUS1 and PTEN in clinical tumor samples.Clinical epigenetics · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundPTEN is an important tumour suppressor gene that is mutated in Cowden syndrome as well as various sporadic cancers. CpG island hypermethylation is another route to tumour suppressor gene inactivation, however, the literature regarding PTEN hypermethylation in cancer is controversial. Furthermore, investigation of the methylation status of the PTEN CpG island is challenging due to sequence homology with the PTEN pseudogene, PTENP1. PTEN shares a CpG island promoter with another gene known as KLLN. Here we present a thorough reinvestigation of the methylation status of the PTEN CpG island in DNA from colorectal, breast, ovarian, glioma, lung and haematological cancer cell lines.
resultsUsing a range of bisulphite-based PCR assays we investigated 6 regions across the PTEN CpG island. We found that regions 1-4 were not methylated in cancer cell lines (0/36). By allelic bisulphite sequencing and pyrosequencing methylation was detected in regions 5 and 6 in colorectal, breast and haematological cancer cell lines. However, methylation detected in this region was associated with the PTENP1 promoter and not the PTEN CpG island.
conclusionsWe show that methylation of the PTEN CpG island is a rare event in cancer cell lines and that apparent methylation most likely originates from homologous regions of the PTENP1 pseudogene promoter. Future studies should utilize assays that reliably discriminate between PTEN and PTENP1 to avoid data misinterpretation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.