ArticleBiophysical journal1990
Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo applications.
Article in Biophysical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Inferences from FRAP data are model dependent: A subdiffusive analysis.Biophysical journal · 2022Article
- Review
- Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion.Journal of mathematical biology · 2021Article
- Article
- Quantitating repair protein accumulation at DNA lesions: Past, present, and future.DNA repair · 2019Review
- Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress.Scientific reports · 2018Article
- Fluorescence recovery after photobleaching investigation of protein transport and exchange in chromatographic media.Journal of chromatography. A · 2014Article
- FRAP in pharmaceutical research: practical guidelines and applications in drug delivery.Pharmaceutical research · 2014Review
- Delivery of molecular and cellular medicine to solid tumors.Advanced drug delivery reviews · 2012Article
- Article
- Simultaneous measurement of anisotropic solute diffusivity and binding reaction rates in biological tissues by FRAP.Annals of biomedical engineering · 2011Article
- Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.Biophysical journal · 2009Article
- Diffusion in brain extracellular space.Physiological reviews · 2008Review
- Inferring the lifetime of endosomal protein complexes by fluorescence recovery after photobleaching.Biophysical journal · 2008Article
- A finite element model for protein transport in vivo.Biomedical engineering online · 2007Article
- Identification of biomolecule mass transport and binding rate parameters in living cells by inverse modeling.Theoretical biology & medical modelling · 2006Article
- Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching.Biophysical journal · 2006Article
- Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins.Biophysical journal · 2006Article
- Analysis of binding reactions by fluorescence recovery after photobleaching.Biophysical journal · 2004Article
- Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes.Molecular and cellular biology · 2004Article
Corrections and comments
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2 authors.
Funding
Abstract
Fluorescence Recovery After Photobleaching (FRAP) has been used extensively in the study of transport and binding in biological media in vitro. The present study adapts and further develops FRAP so that it may be utilized for the in vivo quantification of binding parameters. The technique is validated in vitro by measuring mass transport and binding parameters for the Concanavalin A/Mannose binding system (a diffusion-limited system). The pseudo-equilibrium constant (the product of the equilibrium constant and the total concentration of binding sites) for this system was determined to be 26 +/- 15 which compares favorably with literature values ranging between 16 and 32. The applicability of this technique to measure parameters for monoclonal antibody/antigen interactions in a thin tissue preparation such as the rabbit ear chamber tissue preparation is also examined. Unlike other methods for measuring binding parameters, this is the only technique which has the potential to measure parameters relevant to antibody delivery in vivo. The proposed technique is noninvasive and does not require a priori knowledge of, independent measurement of, or variation in the concentration of binding sites or total concentration of binding species.
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