ArticleInternational journal of molecular sciences2012
Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Review
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Functional Water Networks in Fully Hydrated Photosystem II.Journal of the American Chemical Society · 2022Article
- Best practices for time-resolved serial synchrotron crystallography.Acta crystallographica. Section D, Structural biology · 2022Review
- Engineering Crystal Packing in RNA Structures I: Past and Future Strategies for Engineering RNA Packing in Crystals.Crystals · 2021Article
- Detection of Microcrystals for CryoEM.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin.Acta crystallographica. Section D, Structural biology · 2019Article
- Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography.Acta crystallographica. Section D, Structural biology · 2019Article
- Effects of protein-crystal hydration and temperature on side-chain conformational heterogeneity in monoclinic lysozyme crystals.Acta crystallographica. Section D, Structural biology · 2018Article
- Guidelines for the successful generation of protein-ligand complex crystals.Acta crystallographica. Section D, Structural biology · 2017Review
- A generic protocol for protein crystal dehydration using the HC1b humidity controller.Acta crystallographica. Section D, Structural biology · 2016Article
- Improving diffraction resolution using a new dehydration method.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Crystal Dehydration in Membrane Protein Crystallography.Advances in experimental medicine and biology · 2016Article
- Post-crystallization Improvement of RNA Crystal Diffraction Quality.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Protein Recognition of Gold-Based Drugs: 3D Structure of the Complex Formed When Lysozyme Reacts with Aubipy(c.).ACS medicinal chemistry letters · 2014Article
- Dramatic improvement of crystals of large RNAs by cation replacement and dehydration.Structure (London, England : 1993) · 2014Article
- An evaluation of adhesive sample holders for advanced crystallographic experiments.Acta crystallographica. Section D, Biological crystallography · 2014Article
- IR laser-induced protein crystal transformation.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Membrane protein structure determination - the next generation.Biochimica et biophysica acta · 2014Review
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4 authors.
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Abstract
Serum albumin is one of the most widely studied proteins. It is the most abundant protein in plasma with a typical concentration of 5 g/100 mL and the principal transporter of fatty acids in plasma. While the crystal structures of human serum albumin (HSA) free and in complex with fatty acids, hemin, and local anesthetics have been characterized, no crystallographic models are available on bovine serum albumin (BSA), presumably because of the poor diffraction power of existing hexagonal BSA crystals. Here, the crystallization and diffraction data of a new BSA crystal form, obtained by the hanging drop method using MPEG 5K as precipitating agent, are presented. The crystals belong to space group C2, with unit-cell parameters a = 216.45 Å, b = 44.72 Å, c = 140.18 Å, β = 114.5°. Dehydration was found to increase the diffraction limit of BSA crystals from ~8 Å to 3.2 Å, probably by improving the packing of protein molecules in the crystal lattice. These results, together with a survey of more than 60 successful cases of protein crystal dehydration, confirm that it can be a useful procedure to be used in initial screening as a method of improving the diffraction limits of existing crystals.
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