ArticleInternational journal of molecular sciences2012
Development and characterization of 18 novel EST-SSRs from the western flower Thrips, Frankliniella occidentalis (Pergande).
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
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- Comparison and Phylogenetic Analysis of Chloroplast Genomes of Three Medicinal and EdibleInternational journal of molecular sciences · 2019Article
- Sequencing and Structural Analysis of the Complete Chloroplast Genome of the Medicinal PlantPlants (Basel, Switzerland) · 2019Article
- The complete chloroplast genome sequence of Alpinia oxyphylla Miq. and comparison analysis within the Zingiberaceae family.PloS one · 2019Article
- Complete Chloroplast Genomes fromMolecules (Basel, Switzerland) · 2018Article
- The Complete Amomum kravanh Chloroplast Genome Sequence and Phylogenetic Analysis of the Commelinids.Molecules (Basel, Switzerland) · 2017Article
- RNA-seq of Rice Yellow Stem BorerG3 (Bethesda, Md.) · 2017Article
- Complete Chloroplast Genome Sequence and Phylogenetic Analysis of the Medicinal Plant Artemisia annua.Molecules (Basel, Switzerland) · 2017Article
- Bulk development and stringent selection of microsatellite markers in the western flower thrips Frankliniella occidentalis.Scientific reports · 2016Article
- Development and Characterization of Novel Microsatellite Markers for the Peach Fruit Moth Carposina sasakii (Lepidoptera: Carposinidae) Using Next-Generation Sequencing.International journal of molecular sciences · 2016Article
- Development of 10 microsatellite markers from Pantala flavescens and their applicability in studying genetics diversity.Molecular biology reports · 2015Article
- Temporal Genetic Dynamics of an Invasive Species, Frankliniella occidentalis (Pergande), in an Early Phase of Establishment.Scientific reports · 2015Article
- Developing conversed microsatellite markers and their implications in evolutionary analysis of the Bemisia tabaci complex.Scientific reports · 2014Article
- Article
- Rapid development of 36 polymorphic microsatellite markers for Tetranychus truncatus by transferring from Tetranychus urticae.Experimental & applied acarology · 2013Article
- Isolation and characterization of cross-amplification microsatellite panels for species of Procapra (Bovidae; Antilopinae).International journal of molecular sciences · 2012Article
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5 authors.
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Abstract
The western flower thrips, Frankliniella occidentalis (Pergande), is an invasive species and the most economically important pest within the insect order Thysanoptera. For a better understanding of the genetic makeup and migration patterns of F. occidentalis throughout the world, we characterized 18 novel polymorphic EST-derived microsatellites. The mutational mechanism of these EST-SSRs was also investigated to facilitate the selection of appropriate combinations of markers for population genetic studies. Genetic diversity of these novel markers was assessed in 96 individuals from three populations in China (Harbin, Dali, and Guiyang). The results showed that all these 18 loci were highly polymorphic; the number of alleles ranged from 2 to 15, with an average of 5.50 alleles per locus. The observed (H(O)) and expected (H(E)) heterozygosities ranged from 0.072 to 0.707 and 0.089 to 0.851, respectively. Furthermore, only two locus/population combinations (WFT144 in Dali and WFT50 in Guiyang) significantly deviated from Hardy-Weinberg equilibrium (HWE). Pairwise F(ST) analysis showed a low but significant differentiation (0.026 < F(ST) < 0.032) among all three pairwise population comparisons. Sequence analysis of alleles per locus revealed a complex mutational pattern of these EST-SSRs. Thus, these EST-SSRs are useful markers but greater attention should be paid to the mutational characteristics of these microsatellites when they are used in population genetic studies.
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