ArticleInternational journal of molecular sciences2012
Variation in stability of endogenous reference genes in fallopian tubes and endometrium from healthy and ectopic pregnant women.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Towards deep phenotyping pregnancy: a systematic review on artificial intelligence and machine learning methods to improve pregnancy outcomes.Briefings in bioinformatics · 2021Pooled it
- Selecting Optimal Housekeeping Genes for RT-qPCR in Endometrial Cancer Studies: A Narrative Review.International journal of molecular sciences · 2025Review
- Identification of stable housekeeping genes in mouse liver for studying carbon tetrachloride-induced injury and cellular senescence.Scientific reports · 2024Article
- Validating Reference Gene Expression Stability in Human Ovarian Follicles, Oocytes, Cumulus Cells, Ovarian Medulla, and Ovarian Cortex Tissue.International journal of molecular sciences · 2022Article
- Selection of Endogenous Control Reference Genes for Studies on Type 1 or Type 2 Endometrial Cancer.Scientific reports · 2020Article
- Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models.BioMed research international · 2020Article
- Endocannabinoid regulation in human endometrium across the menstrual cycle.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development.Molecular genetics and genomics : MGG · 2014Article
- Article
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5 authors.
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Abstract
RT-qPCR is commonly employed in gene expression studies in ectopic pregnancy. Most use RN18S1, β-actin or GAPDH as internal controls without validation of their suitability as reference genes. A systematic study of the suitability of endogenous reference genes for gene expression studies in ectopic pregnancy is lacking. The aims of this study were therefore to evaluate the stability of 12 reference genes and suggest those that are stable for use as internal control genes in fallopian tubes and endometrium from ectopic pregnancy and healthy non-pregnant controls. Analysis of the results showed that the genes consistently ranked in the top six by geNorm and NormFinder algorithms, were UBC, GAPDH, CYC1 and EIF4A2 (fallopian tubes) and UBC and ATP5B (endometrium). mRNA expression of NAPE-PLD as a test gene of interest varied between the groups depending on which of the 12 reference genes was used as internal controls. This study demonstrates that arbitrary selection of reference genes for normalisation in RT-qPCR studies in ectopic pregnancy without validation, risk producing inaccurate data and should therefore be discouraged.
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