ArticleCell cycle (Georgetown, Tex.)2012
ATP analog-sensitive Pat1 protein kinase for synchronous fission yeast meiosis at physiological temperature.
Article in Cell cycle (Georgetown, Tex.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Gamete fusion triggers cytosolic functions and P-body recruitment of the RNA-binding protein Mei2 to drive fission yeast zygotic development.Genes & development · 2026Article
- Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.Scientific reports · 2024Article
- CDK phosphorylation of Sfr1 downregulates Rad51 function in late-meiotic homolog invasions.The EMBO journal · 2024Article
- AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity inMicroorganisms · 2024Article
- Sororin is an evolutionary conserved antagonist of WAPL.Nature communications · 2024Article
- Metabolic stress-induced long ncRNA transcription governs the formation of meiotic DNA breaks in the fission yeast fbp1 gene.PloS one · 2024Article
- SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Rec8 Cohesin-mediated Axis-loop chromatin architecture is required for meiotic recombination.Nucleic acids research · 2022Article
- Article
- Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeastCell cycle (Georgetown, Tex.) · 2020Article
- Chromosome-associated RNA-protein complexes promote pairing of homologous chromosomes during meiosis in Schizosaccharomyces pombe.Nature communications · 2019Article
- Destabilization of the replication fork protection complex disrupts meiotic chromosome segregation.Molecular biology of the cell · 2017Article
- Multiple Duties for Spindle Assembly Checkpoint Kinases in Meiosis.Frontiers in cell and developmental biology · 2017Review
- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1.BMC plant biology · 2016Article
- Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.PLoS genetics · 2016Article
- A single cyclin-CDK complex is sufficient for both mitotic and meiotic progression in fission yeast.Nature communications · 2015Article
- DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.Nucleic acids research · 2014Article
- Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast.Cell cycle (Georgetown, Tex.) · 2014Article
- Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.Nature protocols · 2014Article
Corrections and comments
- Commented on by
- Commented on by
- Commented on by
Authors and funding
4 authors at 3 institutions in 4 countries.
Funding
Abstract
To study meiosis, synchronous cultures are often indispensable, especially for physical analyses of DNA and proteins. A temperature-sensitive allele of the Pat1 protein kinase (pat1-114) has been widely used to induce synchronous meiosis in the fission yeast Schizosaccharomyces pombe, but pat1-114-induced meiosis differs from wild-type meiosis, and some of these abnormalities might be due to higher temperature needed to inactivate the Pat1 kinase. Here, we report an ATP analog-sensitive allele of Pat1 [Pat1(L95A), designated pat1-as2] that can be used to generate synchronous meiotic cultures at physiological temperature. In pat1-as2 meiosis, chromosomes segregate with higher fidelity, and spore viability is higher than in pat1-114 meiosis, although recombination is lower by a factor of 2-3 in these mutants than in starvation-induced pat1(+) meiosis. Addition of the mat-Pc gene improved chromosome segregation and spore viability to nearly the level of starvation-induced meiosis. We conclude that pat1-as2 mat-Pc cells offer synchronous meiosis with most tested properties similar to those of wild-type meiosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.