ArticleToxicology2012
Assessing the relevance of in vitro studies in nanotoxicology by examining correlations between in vitro and in vivo data.
Article in Toxicology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- A newFrontiers in toxicology · 2026Article
- Application of in vitro pulmonary models for hazard screening of silica particles.Archives of toxicology · 2025Article
- Interactions Between Silver Nanoparticles and Culture Medium Biomolecules with Dose and Time Dependencies.Journal of fluorescence · 2025Article
- Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis.Particle and fibre toxicology · 2023Article
- The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.Nanomaterials (Basel, Switzerland) · 2023Review
- Predicting nanomaterials pulmonary toxicity in animals by cell culture models: Achievements and perspectives.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Approach towardEnvironmental science & technology · 2022Article
- Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome.International journal of environmental research and public health · 2022Article
- The in silico and in vitro analysis of donepezil derivatives for Anopheles acetylcholinesterase inhibition.PloS one · 2022Article
- Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner.Nanomaterials (Basel, Switzerland) · 2021Article
- Oxidative stress pathways of air pollution mediated toxicity: Recent insights.Redox biology · 2020Review
- Effects of Ultrasonic Dispersion Energy on the Preparation of Amorphous SiO₂ Nanomaterials for In Vitro Toxicity Testing.Nanomaterials (Basel, Switzerland) · 2018Article
- The asbestos-carbon nanotube analogy: An update.Toxicology and applied pharmacology · 2018Review
- Combustion-derived particles inhibit in vitro human lung fibroblast-mediated matrix remodeling.Journal of nanobiotechnology · 2018Article
- Characterization of Nanoparticle Batch-To-Batch Variability.Nanomaterials (Basel, Switzerland) · 2018Article
- Effect of Particulate Matter Air Pollution on Cardiovascular Oxidative Stress Pathways.Antioxidants & redox signaling · 2018Review
- In Vitro and In Vivo Short-Term Pulmonary Toxicity of Differently Sized Colloidal Amorphous SiO₂.Nanomaterials (Basel, Switzerland) · 2018Article
- Ins and Outs in Environmental and Occupational Safety Studies of Asthma and Engineered Nanomaterials.ACS nano · 2017Article
- The roles of surface chemistry, dissolution rate, and delivered dose in the cytotoxicity of copper nanoparticles.Nanoscale · 2017Article
- Mechanisms allowing protein delivery in nasal mucosa using NPL nanoparticles.Journal of controlled release : official journal of the Controlled Release Society · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
There is an urgent need for in vitro screening assays to evaluate nanoparticle (NP) toxicity. However, the relevance of in vitro assays is still disputable. We administered doses of TiO(2) NPs of different sizes to alveolar epithelial cells in vitro and the same NPs by intratracheal instillation in rats in vivo to examine the correlation between in vitro and in vivo responses. The correlations were based on toxicity rankings of NPs after adopting NP surface area as dose metric, and response per unit surface area as response metric. Sizes of the anatase TiO(2) NPs ranged from 3 to 100 nm. A cell-free assay for measuring reactive oxygen species (ROS) was used, and lactate dehydrogenase (LDH) release, and protein oxidation induction were the in vitro cellular assays using a rat lung Type I epithelial cell line (R3/1) following 24 h incubation. The in vivo endpoint was number of PMNs in bronchoalveolar lavage fluid (BALF) after exposure of rats to the NPs via intratracheal instillation. Slope analyses of the dose response curves shows that the in vivo and in vitro responses were well correlated. We conclude that using the approach of steepest slope analysis offers a superior method to correlate in vitro with in vivo results of NP toxicity and for ranking their toxic potency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.