ArticleNature methods2012
Segregation of molecules at cell division reveals native protein localization.
Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 195 papers.
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Who cites it
195 citing papers in PubMed.
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- Glycogen phase separation drives macromolecular rearrangement and asymmetric division inbioRxiv : the preprint server for biology · 2025Article
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- A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging.Science advances · 2025Article
- Structural insights into the fusion of annexin A5 and fluorescent proteins generating hundredfold differentiated binding affinities to phosphatidylserine.Protein science : a publication of the Protein Society · 2025Article
- iPAR: a new reporter for eukaryotic cytoplasmic protein aggregation.BMC methods · 2025Article
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- Analysis of Fluorescent Proteins for Observing Single Gene Locus in a Live and FixedThe journal of physical chemistry. B · 2024Article
- A vector system for single and tandem expression of cloned genes and multi-colour fluorescent tagging inMicrobiology (Reading, England) · 2024Article
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- VirB, a key transcriptional regulator ofmBio · 2023Article
135 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
We introduce a nonintrusive method exploiting single-cell variability after cell division to validate protein localization. We found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers. Retagging five other reportedly foci-forming proteins with the most monomeric fluorescent protein tested suggests that the foci were caused by the fluorescent tags.
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