ArticleMicrovascular research2012
A novel and simple method for culturing pericytes from mouse brain.
Article in Microvascular research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 118 citations in OpenAlex.
- Patient-Derived Procoagulant Breast Fibroblasts Expressing Tissue Factor Promote Breast Cancer Cell Migration.Journal of mammary gland biology and neoplasia · 2026Article
- Eppur si muove: the dynamic brain pericyte.Fluids and barriers of the CNS · 2025Review
- Pericyte loss: a key factor inducing brain Aβ40 accumulation and neuronal degeneration in cerebral amyloid angiopathy.Experimental brain research · 2025Article
- Kindlin-2 Deletion in Mural Cells Leads to Vascular Instability.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Review
- Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1.Nature communications · 2024Article
- A switch in the pathway of TRPC3-mediated calcium influx into brain pericytes contributes to capillary spasms after subarachnoid hemorrhage.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2024Article
- Isolation methods and characterization of primary rat neurovascular cells.Journal of biological engineering · 2024Article
- Brain pericytes in culture display diverse morphological and functional phenotypes.Cell biology and toxicology · 2023Article
- Bone-derived PDGF-BB drives brain vascular calcification in male mice.The Journal of clinical investigation · 2023Article
- Pericytes are protective in experimental pneumococcal meningitis through regulating leukocyte infiltration and blood-brain barrier function.Journal of neuroinflammation · 2023Article
- Development of an Animal Model for Traumatic Brain Injury Augmentation of Heterotopic Ossification in Response to Local Injury.Biomedicines · 2023Article
- Unique expression of the atypical mitochondrial subunit NDUFA4L2 in cerebral pericytes fine tunes HIF activity in response to hypoxia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- Cultured brain pericytes adopt an immature phenotype and require endothelial cells for expression of canonical markers and ECM genes.Frontiers in cellular neuroscience · 2023Article
- CNS Pericytes Modulate Local T Cell Infiltration in EAE.International journal of molecular sciences · 2022Article
- Mural Cell SRF Controls Pericyte Migration, Vessel Patterning and Blood Flow.Circulation research · 2022Article
- Iptakalim improves cerebral microcirculation in mice after ischemic stroke by inhibiting pericyte contraction.Acta pharmacologica Sinica · 2022Article
- Biosynthetic Gas Vesicles Combined with Focused Ultrasound for Blood-Brain Barrier Opening.International journal of nanomedicine · 2022Article
- Approaches for the isolation and long-term expansion of pericytes from human and animal tissues.Frontiers in cardiovascular medicine · 2022Article
- Pericyte Insulin Receptors Modulate Retinal Vascular Remodeling and Endothelial Angiopoietin Signaling.Endocrinology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Pericytes play critical roles in the development, maturation and remodeling of blood vessels, and in the central nervous system (CNS), evidence suggests that pericytes also regulate blood flow and form an integral part of the blood-brain barrier. The study of this important cell type has been hampered by the lack of any pericyte-specific marker and by the difficulty of culturing pericytes in adequate numbers to high purity. Here we present a novel yet simple approach to isolate and culture large numbers of pericytes from the mouse CNS that nevertheless leads to very pure pericyte cultures. In our method, vascular cells obtained from adult mice brains are cultured initially under conditions optimized for endothelial cells, but after two passages switched to a medium optimized for pericyte growth. After growing the cells for 1-2 additional passages we obtained a largely homogeneous population of cells that expressed the pericyte markers NG2, PDGFβ-receptor, and CD146, but were negative for markers of endothelial cells (CD31), microglia (Mac-1) and astrocytes (GFAP). Under these conditions, pericytes could be grown to high passage number, and were maintained highly pure and largely undifferentiated, as determined by antigen expression profile and low levels of α-SMA expression, a marker of pericyte differentiation. Furthermore, switching the cells from pericyte medium into DMEM containing 10% FBS promoted α-SMA expression, demonstrating that high passage pericytes could still differentiate. Thus, we provide an alternative approach to the culture of CNS pericytes that is easy to establish and provides large numbers of highly pure pericytes for extended periods of time. This system should provide others working in the pericyte field with a useful additional tool to study the behavior of this fascinating cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.