ArticleMicrobes and infection2012
Transcriptome analysis of HeLa cells response to Brucella melitensis infection: a molecular approach to understand the role of the mucosal epithelium in the onset of the Brucella pathogenesis.
Article in Microbes and infection, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Brucellosis: Bacteriology, pathogenesis, epidemiology and role of the metallophores in virulence: a review.Frontiers in cellular and infection microbiology · 2025Review
- Cell and Tissue Tropism of Brucella spp.Infection and immunity · 2023Review
- Comparative Review of Brucellosis in Small Domestic Ruminants.Frontiers in veterinary science · 2022Review
- Scenario of pathogenesis and socioeconomic burden of human brucellosis in Saudi Arabia.Saudi journal of biological sciences · 2021Review
- Bacterial Infections Affect Male Fertility: A Focus on the Oxidative Stress-Autophagy Axis.Frontiers in cell and developmental biology · 2021Review
- Characterization of circulating miRNA signature in water buffaloes (Bubalus bubalis) during Brucella abortus infection and evaluation as potential biomarkers for non-invasive diagnosis in vaginal fluid.Scientific reports · 2019Article
- Coincidence cloning recovery of Brucella melitensis RNA from goat tissues: advancing the in vivo analysis of pathogen gene expression in brucellosis.BMC molecular biology · 2018Article
- Systems Biology Analysis of TemporalFrontiers in microbiology · 2017Article
- Full-Length cDNA Cloning, Molecular Characterization and Differential Expression Analysis of Lysophospholipase I from Ovis aries.International journal of molecular sciences · 2016Article
- Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions.The American journal of pathology · 2015Review
- The impact of "omic" and imaging technologies on assessing the host immune response to biodefence agents.Journal of immunology research · 2014Review
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Brucella spp. infect hosts primarily by adhering and penetrating mucosal surfaces, however the initial molecular phenomena of this host:pathogen interaction remain poorly understood. We hypothesized that characterizing the epithelial-like human HeLa cell line molecular response to wild type Brucella melitensis infection would help to understand the role of the mucosal epithelium at the onset of the Brucella pathogenesis. RNA samples from B. melitensis-infected HeLa cells were taken at 4 and 12 h of infection and hybridized in a cDNA microarray. The analysis using a dynamic Bayesian network modeling approach (DBN) identified several pathways, biological processes, cellular components and molecular functions altered due to infection at 4 h p.i., but almost none at 12 h p.i. The in silico modeling results were experimentally tested by knocking down the expression of MAPK1 by siRNA technology. MAPK1-siRNA transfected cell cultures decreased the internalization and impaired the intracellular replication of the pathogen in HeLa cells after 4 h p.i. DBN analysis provides important insights into the role of the epithelial cells response to Brucella infection and guide research to novel mechanisms identification.
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Registered trials
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