ArticleCurrent biology : CB2012
EB1-recruited microtubule +TIP complexes coordinate protrusion dynamics during 3D epithelial remodeling.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Microtubule Engagement with Taxane Is Altered in Taxane-Resistant Gastric Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2020Trial
- Mechanical force locally damages, remodels, and stabilizes the lattice of spindle microtubules.Current biology : CB · 2026Article
- Tuning collagen nonlinear mechanics with interpenetrating networks drives adaptive cellular phenotypes in three dimensions.Science advances · 2025Article
- Microtubule regulation in cancer cells.Frontiers in cell and developmental biology · 2025Article
- Modulation of the cytoskeleton for cancer therapy.Frontiers in cell and developmental biology · 2025Article
- Computational analysis of treatment resistant cancer cells.Frontiers in cell and developmental biology · 2025Article
- Characterization of human Ccser2 as a protein tracking the plus-ends of microtubules.BMC research notes · 2023Article
- Increased Microtubule Growth Triggered by Microvesicle-mediated Paracrine Signaling is Required for Melanoma Cancer Cell Invasion.Cancer research communications · 2022Article
- Manipulation of the Tubulin Code Alters Directional Cell Migration and Ciliogenesis.Frontiers in cell and developmental biology · 2022Article
- Intrinsic Mechanisms Regulating Neuronal Migration in the Postnatal Brain.Frontiers in cellular neuroscience · 2020Review
- Microtubule Plus End Dynamics - Do We Know How Microtubules Grow?: Cells boost microtubule growth by promoting distinct structural transitions at growing microtubule ends.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
- CLIP-170 spatially modulates receptor tyrosine kinase recycling to coordinate cell migration.Traffic (Copenhagen, Denmark) · 2019Article
- Actin-microtubule crosstalk in cell biology.Nature reviews. Molecular cell biology · 2019Review
- EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Local control of intracellular microtubule dynamics by EB1 photodissociation.Nature cell biology · 2018Article
- Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures.Scientific reports · 2017Article
- Review
- The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure inJournal of cell science · 2017Article
- Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its redeployment to non-centrosomal microtubule organizing centres.Open biology · 2017Article
- Role of End Binding Protein-1 in endothelial permeability response to barrier-disruptive and barrier-enhancing agonists.Cellular signalling · 2017Article
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2 authors.
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Abstract
backgroundEpithelial remodeling, in which apical-basal polarized cells switch to a migratory phenotype, plays a central role in development and disease of multicellular organisms. Although dynamic microtubules (MTs) are required for directed migration on flat surfaces, how MT dynamics are controlled or contribute to epithelial remodeling in a more physiological three-dimensional (3D) environment is not understood. We use confocal live-cell imaging to analyze MT function and dynamics during 3D epithelial morphogenesis and remodeling of polarized Madin-Darby canine kidney epithelial cells that undergo partial epithelial-to-mesenchymal transition in response to hepatocyte growth factor (HGF).
resultsWe find that HGF treatment increases MT growth rate before morphological changes are evident and that large numbers of MTs grow into HGF-induced cell extensions independent of centrosome reorientation. Using lentivirus-mediated small hairpin RNA, we demonstrate that EB1, an adaptor protein that mediates recruitment of numerous other +TIP proteins to growing MT plus ends, is required for this HGF-induced MT reorganization. We further show that protrusion and adhesion dynamics are disorganized and that vesicular trafficking to the tip of HGF-induced cell extensions is disrupted in EB1-depleted cells.
conclusionsWe conclude that EB1-mediated interactions with growing MTs are important to coordinate cell shape changes and directed migration into the surrounding extracellular matrix during epithelial remodeling in a physiological 3D environment. In contrast, EB1 is not required for the establishment or maintenance of apical-basal cell polarity, suggesting different functions of +TIPs and MTs in different types of cell polarity.
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