ArticleAnalytical chemistry2012
Channel surface patterning of alternating biomimetic protein combinations for enhanced microfluidic tumor cell isolation.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- The integrated on-chip isolation and detection of circulating tumour cells.Sensors & diagnostics · 2024Review
- Magnetic Particles for CTC Enrichment.Cancers · 2020Review
- Liquid biopsy in pancreatic cancer: the beginning of a new era.Oncotarget · 2018Review
- Multivalent Binding and Biomimetic Cell Rolling Improves the Sensitivity and Specificity of Circulating Tumor Cell Capture.Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Integration of biomimicry and nanotechnology for significantly improved detection of circulating tumor cells (CTCs).Advanced drug delivery reviews · 2018Review
- E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Highly-sensitive capture of circulating tumor cells using micro-ellipse filters.Scientific reports · 2017Article
- Microfluidic devices to enrich and isolate circulating tumor cells.Lab on a chip · 2015Review
- Rational Design of Materials Interface for Efficient Capture of Circulating Tumor Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2015Article
- Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies.Analytical chemistry · 2014Article
- Microfluidic blood cell sorting: now and beyond.Small (Weinheim an der Bergstrasse, Germany) · 2014Review
- Review
- Recent advances in microfluidic cell separations.The Analyst · 2013Review
- Recent advances in nanotechnology-based detection and separation of circulating tumor cells.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Here, we report a new method for multicomponent protein patterning in a microchannel and also a technique for improving immunoaffinity-based circulating tumor cell (CTC) capture by patterning regions of alternating adhesive proteins using the new method. The first of two proteins, antiepithelial cell adhesion molecule (anti-EpCAM), provides the specificity for CTC capture. The second, E-selectin, increases CTC capture under shear. Patterning regions with and without E-selectin allows captured leukocytes, which also bind E-selectin and are unwanted impurities in CTC isolation, to roll a short distance and detach from the capture surface. This reduces leukocyte capture by up to 82%. The patterning is combined with a leukocyte elution step in which a calcium chelating buffer effectively deactivates E-selectin so that leukocytes may be rinsed away 60% more efficiently than with a buffer containing calcium. The alternating patterning of this biomimetic protein combination, used in conjunction with the elution step, reduces capture of leukocytes while maintaining a high tumor cell capture efficiency that is up to 1.9 times higher than the tumor cell capture efficiency of a surface with only anti-EpCAM. The new patterning technique described here does not require mask alignment and can be used to spatially control the immobilization of any two proteins or protein mixtures inside a sealed microfluidic channel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.