ArticleNature protocols2012
Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed.
- Polymerase Chain Reaction Chips for Biomarker Discovery and Validation in Drug Development.Micromachines · 2025Review
- Human monocyte subtype expression of neuroinflammation- and regeneration-related genes is linked to age and sex.PloS one · 2024Article
- Transcription Factor-Forced Astrocytic Differentiation Impairs Human Glioblastoma Growth In Vitro and In Vivo.Molecular cancer therapeutics · 2023Article
- RNA cytometry of single-cells using semi-permeable microcapsules.Nucleic acids research · 2023Article
- Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry.Biosensors · 2022Article
- Single cell multi-miRNAs quantification with hydrogel microbeads for liver cancer cell subtypes discrimination.Chemical science · 2022Article
- Challenges and approaches to studying pore-forming proteins.Biochemical Society transactions · 2021Review
- Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection.Scientific reports · 2021Article
- Review
- Article
- Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene.Micromachines · 2020Article
- Multivariate patterning of human pluripotent cells under perfusion reveals critical roles of induced paracrine factors in kidney organoid development.Science advances · 2020Article
- Droplets as Carriers for Flexible Electronic Devices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders.Nature communications · 2019Article
- Proteasome-Dependent Regulation of Distinct Metabolic States During Long-Term Culture of Human iPSC-Derived Cardiomyocytes.Circulation research · 2019Article
- Segmented Filamentous Bacteria Induce Divergent Populations of Antigen-Specific CD4 T Cells in the Small Intestine.Molecules and cells · 2019Article
- Microfluidic-Based Single-Cell Study: Current Status and Future Perspective.Molecules (Basel, Switzerland) · 2018Review
- Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes.Genome medicine · 2018Article
- Using single-cell genomics to understand developmental processes and cell fate decisions.Molecular systems biology · 2018Review
- Single-Cell RT-PCR in Microfluidic Droplets with Integrated Chemical Lysis.Analytical chemistry · 2018Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample collection. We present here a high-content microfluidic real-time platform as a powerful tool for comparatively investigating the regulation of developmental processes in single cells. This approach overcomes the limitations involving heterogeneous cell populations and sample amounts, and may shed light on differential regulation of gene expression in normal versus disease-related contexts. Furthermore, high-throughput single-cell qRT-PCR provides a standardized, comparative assay for in-depth analysis of the mechanisms underlying human pluripotent stem cell self-renewal and differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.