ArticleJournal of the American Chemical Society2012
Biochemical and structural characterization of germicidin synthase: analysis of a type III polyketide synthase that employs acyl-ACP as a starter unit donor.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Unveiling the genomes and secondary metabolomes ofFrontiers in microbiology · 2026Article
- Elucidating the Iterative Elongation Mechanism in a Type III Polyketide Synthase.Journal of the American Chemical Society · 2025Article
- Improved polyketide production in C. glutamicum by preventing propionate-induced growth inhibition.Nature metabolism · 2023Article
- The virulome ofMicrobial genomics · 2022Article
- Genome Mining and Evolutionary Analysis Reveal Diverse Type III Polyketide Synthase Pathways in Cyanobacteria.Genome biology and evolution · 2021Article
- Expression of genes of the Pho regulon is altered in Streptomyces coelicolor.Scientific reports · 2020Article
- Article
- Biosynthesis of Polyketides inMicroorganisms · 2019Review
- Genome mining reveals uncommon alkylpyrones as type III PKS products from myxobacteria.Journal of industrial microbiology & biotechnology · 2019Article
- Novel Type III Polyketide Synthases Biosynthesize Methylated Polyketides in Mycobacterium marinum.Scientific reports · 2018Article
- Article
- Non-targeted metabolomics unravels a media-dependent prodiginines production pathway in Streptomyces coelicolor A3(2).PloS one · 2018Article
- Germicidins H-J from Streptomyces sp. CB00361.The Journal of antibiotics · 2017Article
- Secondary Metabolites Produced during the Germination ofFrontiers in microbiology · 2017Article
- Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66.Microbial cell factories · 2016Article
- Exploiting the Biosynthetic Potential of Type III Polyketide Synthases.Molecules (Basel, Switzerland) · 2016Review
- Assembly line termination in cylindrocyclophane biosynthesis: discovery of an editing type II thioesterase domain in a type I polyketide synthase.Chemical science · 2015Article
- A novel and widespread class of ketosynthase is responsible for the head-to-head condensation of two acyl moieties in bacterial pyrone biosynthesis.Beilstein journal of organic chemistry · 2015Article
- Acyl-ACP substrate recognition in Burkholderia mallei BmaI1 acyl-homoserine lactone synthase.Biochemistry · 2014Article
- Structure of a modular polyketide synthase.Nature · 2014Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Germicidin synthase (Gcs) from Streptomyces coelicolor is a type III polyketide synthase (PKS) with broad substrate flexibility for acyl groups linked through a thioester bond to either coenzyme A (CoA) or acyl carrier protein (ACP). Germicidin synthesis was reconstituted in vitro by coupling Gcs with fatty acid biosynthesis. Since Gcs has broad substrate flexibility, we directly compared the kinetic properties of Gcs with both acyl-ACP and acyl-CoA. The catalytic efficiency of Gcs for acyl-ACP was 10-fold higher than for acyl-CoA, suggesting a strong preference toward carrier protein starter unit transfer. The 2.9 Å germicidin synthase crystal structure revealed canonical type III PKS architecture along with an unusual helical bundle of unknown function that appears to extend the dimerization interface. A pair of arginine residues adjacent to the active site affect catalytic activity but not ACP binding. This investigation provides new and surprising information about the interactions between type III PKSs and ACPs that will facilitate the construction of engineered systems for production of novel polyketides.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.