ArticleHuman gene therapy2012
Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays.
Article in Human gene therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Dissolving microneedles for nucleic acid delivery: A systematic search, review, and data synthesis.Acta biomaterialia · 2025Pooled it
- Biomaterial-based gene therapy.MedComm · 2023Review
- The most promising microneedle device: present and future of hyaluronic acid microneedle patch.Drug delivery · 2022Review
- Transdermal delivery for gene therapy.Drug delivery and translational research · 2022Review
- Fractional Laser-Mediated siRNA Delivery for Mitigating Psoriasis-like Lesions via IL-6 Silencing.Molecular therapy. Nucleic acids · 2020Article
- Smart Microneedles for Therapy and Diagnosis.Research (Washington, D.C.) · 2020Review
- Formulation, characterization and evaluation of mRNA-loaded dissolvable polymeric microneedles (RNApatch).Scientific reports · 2018Article
- Prognostic impact of carbohydrate sulfotransferase 15 in patients with pancreatic ductal adenocarcinoma.Oncology letters · 2017Article
- Transdermal Delivery of siRNA through Microneedle Array.Scientific reports · 2016Article
- Non-Invasive Intravital Imaging of siRNA-Mediated Mutant Keratin Gene Repression in Skin.Molecular imaging and biology · 2016Article
- Microneedles As a Delivery System for Gene Therapy.Frontiers in pharmacology · 2016Review
- Connexin hemichannels influence genetically determined inflammatory and hyperproliferative skin diseases.Pharmacological research · 2015Article
- Short-interference RNAs: becoming medicines.EXCLI journal · 2015Article
- The Role of CD44 in Disease Pathophysiology and Targeted Treatment.Frontiers in immunology · 2015Review
- In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Gene Silencing in Skin After Deposition of Self-Delivery siRNA With a Motorized Microneedle Array Device.Molecular therapy. Nucleic acids · 2013Article
- Silencing human genetic diseases with oligonucleotide-based therapies.Human genetics · 2013Review
- Gene silencing following siRNA delivery to skin via coated steel microneedles: In vitro and in vivo proof-of-concept.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- RNA interference trigger variants: getting the most out of RNA for RNA interference-based therapeutics.Nucleic acid therapeutics · 2012Review
- RNA interference for the treatment of papillomavirus disease.The open virology journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Treatment of skin disorders with short interfering RNA (siRNA)-based therapeutics requires the development of effective delivery methodologies that reach target cells in affected tissues. Successful delivery of functional siRNA to the epidermis requires (1) crossing the stratum corneum, (2) transfer across the keratinocyte membrane, followed by (3) incorporation into the RNA-induced silencing complex. We have previously demonstrated that treatment with microneedle arrays loaded with self-delivery siRNA (sd-siRNA) can achieve inhibition of reporter gene expression in a transgenic mouse model. Furthermore, treatment of human cultured epidermal equivalents with sd-siRNA resulted in inhibition of target gene expression. Here, we demonstrate inhibition of CD44, a gene that is uniformly expressed throughout the epidermis, by sd-siRNA both in vitro (cultured human epidermal skin equivalents) and in vivo (full-thickness human skin equivalents xenografted on immunocompromised mice). Treatment of human skin equivalents with CD44 sd-siRNA markedly decreased CD44 mRNA levels, which led to a reduction of the target protein as confirmed by immunodetection in epidermal equivalent sections with a CD44-specific antibody. Taken together, these results demonstrate that sd-siRNA, delivered by microneedle arrays, can reduce expression of a targeted endogenous gene in a human skin xenograft model.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.