ArticleJournal of the American Chemical Society2012
Lipid metabolite profiling identifies desmosterol metabolism as a new antiviral target for hepatitis C virus.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 45 citations in OpenAlex.
- Chemical Inhibition of Sterol Biosynthesis.Biomolecules · 2024Pooled it
- Investigation of 7-dehydrocholesterol reductase pathway to elucidate off-target prenatal effects of pharmaceuticals: a systematic review.The pharmacogenomics journal · 2016Pooled it
- Article
- Zika virus non-structural protein 4B interacts with DHCR7 to facilitate viral infection.Virologica Sinica · 2023Article
- Dose-Response Effects of 7-Dehydrocholesterol Reductase Inhibitors on Sterol Profiles and Vesicular Stomatitis Virus Replication.ACS pharmacology & translational science · 2022Article
- Metabolite discovery: Biochemistry's scientific driver.Cell metabolism · 2022Review
- Hepatitis C virus NS3-4A protease regulates the lipid environment for RNA replication by cleaving host enzyme 24-dehydrocholesterol reductase.The Journal of biological chemistry · 2020Article
- IL-17 signaling in steatotic hepatocytes and macrophages promotes hepatocellular carcinoma in alcohol-related liver disease.Journal of hepatology · 2020Article
- Discovery of host-targeted covalent inhibitors of dengue virus.Antiviral research · 2017Article
- Apolipoprotein B100 is required for hepatitis C infectivity and Mipomersen inhibits hepatitis C.World journal of gastroenterology · 2016Article
- Desmosterol Increases Lipid Bilayer Fluidity during Hepatitis C Virus Infection.ACS infectious diseases · 2016Article
- Hepatitis C Virus Selectively Alters the Intracellular Localization of Desmosterol.ACS chemical biology · 2016Article
- An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway.PLoS biology · 2016Article
- Targeting host lipid synthesis and metabolism to inhibit dengue and hepatitis C viruses.Antiviral research · 2015Review
- Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells.Journal of virology · 2015Article
- Targeting of the hydrophobic metabolome by pathogens.Traffic (Copenhagen, Denmark) · 2015Review
- Targeting cellular squalene synthase, an enzyme essential for cholesterol biosynthesis, is a potential antiviral strategy against hepatitis C virus.Journal of virology · 2015Article
- The bioactive lipid 4-hydroxyphenyl retinamide inhibits flavivirus replication.Antimicrobial agents and chemotherapy · 2015Article
- Signaling regulates activity of DHCR24, the final enzyme in cholesterol synthesis.Journal of lipid research · 2014Article
- Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Hepatitis C virus (HCV) infection has been clinically associated with serum lipid abnormalities, yet our understanding of the effects of HCV on host lipid metabolism and conversely the function of individual lipids in HCV replication remains incomplete. Using liquid chromatography-mass spectrometry metabolite profiling of the HCV JFH1 cell culture infection model, we identified a significant steady-state accumulation of desmosterol, an immediate precursor to cholesterol. Pharmacological inhibition or RNAi-mediated depletion of DHCR7 significantly reduced steady-state HCV protein expression and viral genomic RNA. Moreover, this effect was reversed when cultures were supplemented with exogenous desmosterol. Together, these observations suggest an intimate connection between HCV replication and desmosterol homeostasis and that the enzymes responsible for synthesis of desmosterol may be novel targets for antiviral design.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.