ArticlePLoS neglected tropical diseases2012
A pilot study on developing mucosal vaccine against alveolar echinococcosis (AE) using recombinant tetraspanin 3: Vaccine efficacy and immunology.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Unveiling the promise: Exosomes as game-changers in anti-infective therapy.Exploration (Beijing, China) · 2024Review
- Molecular characterization and B-cell epitope analysis of the TSP11 gene inParasitology · 2024Article
- Current status and future prospects ofVeterinary and animal science · 2024Review
- Design of a novel EmTSP-3 and EmTIP based multi-epitope vaccine againstFrontiers in immunology · 2024Article
- Global profiling of the proteome, phosphoproteome, and N-glycoproteome of protoscoleces and adult worms ofFrontiers in veterinary science · 2023Article
- Prediction and identification of epitopes in the Echinococcus multilocularis thrombospondin 3 antigen.Technology and health care : official journal of the European Society for Engineering and Medicine · 2022Article
- Helminth extracellular vesicles: Interactions with the host immune system.Molecular immunology · 2021Review
- Identification of Different Extracellular Vesicles in the Hydatid Fluid ofFrontiers in immunology · 2021Article
- Molecular Characterization of a Tetraspanin TSP11 Gene inFrontiers in veterinary science · 2021Article
- Comparative Transcriptome Analyses of the Developmental Stages ofFrontiers in veterinary science · 2021Article
- Extracellular vesicles: new targets for vaccines against helminth parasites.International journal for parasitology · 2020Review
- Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus.Parasites & vectors · 2020Article
- Recombinant Opisthorchis viverrini tetraspanin expressed in Pichia pastoris as a potential vaccine candidate for opisthorchiasis.Parasitology research · 2019Article
- Metazoan Parasite Vaccines: Present Status and Future Prospects.Frontiers in cellular and infection microbiology · 2018Review
- Quantitative multiplexed proteomics of Taenia solium cysts obtained from the skeletal muscle and central nervous system of pigs.PLoS neglected tropical diseases · 2017Article
- Immunization of sheep againstVeterinary world · 2017Article
- Exosome-like vesicles of helminths: implication of pathogenesis and vaccine development.Annals of translational medicine · 2017Article
- Host parasite communications-Messages from helminths for the immune system: Parasite communication and cell-cell interactions.Molecular and biochemical parasitology · 2016Review
- Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.PLoS neglected tropical diseases · 2016Article
- Carcinogenic Liver Fluke Secretes Extracellular Vesicles That Promote Cholangiocytes to Adopt a Tumorigenic Phenotype.The Journal of infectious diseases · 2015Article
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Authors and funding
11 authors at 8 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe have previously evaluated the vaccine efficacies of seven tetraspanins of Echinococcus multilocularis (Em-TSP1-7) against alveolar echinococcosis (AE) by subcutaneous (s.c.) administration with Freund's adjuvant. Over 85% of liver cyst lesion number reductions (CLNR) were achieved by recombinant Em-TSP1 (rEm-TSP1) and -TSP3 (rEm-TSP3). However, to develop an efficient and safe human vaccine, the efficacy of TSP mucosal vaccines must be thoroughly evaluated. METHODOLOGY/PRINCIPAL
findingsrEm-TSP1 and -TSP3 along with nontoxic CpG ODN (CpG oligodeoxynucleotides) adjuvant were intranasally (i.n.) immunized to BALB/c mice and their vaccine efficacies were evaluated by counting liver CLNR (experiment I). 37.1% (p < 0.05) and 62.1% (p < 0.001) of CLNR were achieved by these two proteins, respectively. To study the protection-associated immune responses induced by rEm-TSP3 via different immunization routes (i.n. administration with CpG or s.c. immunization with Freund's adjuvant), the systemic and mucosal antibody responses were detected by ELISA (experiment II). S.c. and i.n. administration of rEm-TSP3 achieved 81.9% (p < 0.001) and 62.8% (p < 0.01) CLNR in the liver, respectively. Both the immunization routes evoked strong serum IgG, IgG1 and IgG2α responses; i.n. immunization induced significantly higher IgA responses in nasal cavity and intestine compared with s.c. immunization (p < 0.001). Both immunization routes induced extremely strong liver IgA antibody responses (p < 0.001). The Th1 and Th2 cell responses were assessed by examining the IgG1/IgG2α ratio at two and three weeks post-immunization. S.c. immunization resulted in a reduction in the IgG1/IgG2α ratio (Th1 tendency), whereas i.n. immunization caused a shift from Th1 to Th2. Moreover, immunohistochemistry showed that Em-TSP1 and -TSP3 were extensively located on the surface of E. multilocularis cysts, protoscoleces and adult worms with additional expression of Em-TSP3 in the inner part of protoscoleces and oncospheres.
conclusionsOur study indicated that i.n. administration of rEm-TSP3 with CpG is able to induce both systemic and local immune responses and thus provides significant protection against AE.
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