ArticlePloS one2012
Involvement of iron in biofilm formation by Staphylococcus aureus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
62 citing papers in PubMed, 138 citations in OpenAlex.
- Biological characteristics of a menadione-dependent Staphylococcus aureus small colony variant (SCV) from bovine mastitis and heme promotion of biofilm formation.Archives of microbiology · 2026Article
- FeBiofilm · 2026Review
- Staphylococcus aureus: Antimicrobial resistance, quorum sensing, and antibiofilm approaches.European journal of microbiology & immunology · 2025Review
- Nanoflowers: Smart Molecules for Biofilm Management.Applied biochemistry and biotechnology · 2025Review
- Iron dictates the growth, biofilm formation, and virulence ofFrontiers in microbiology · 2025Article
- Pulcherriminic acid relay; aFrontiers in cellular and infection microbiology · 2025Review
- The adaptability ofFrontiers in cellular and infection microbiology · 2025Review
- Antimicrobial activity of iron-depriving pyoverdines against human opportunistic pathogens.eLife · 2024Article
- Radiotracers for in situ infection imaging: Experimental considerations for in vitro microbial uptake of gallium-68-labeled siderophores.Diagnostic microbiology and infectious disease · 2024Article
- Bacterial single-cell RNA sequencing captures biofilm transcriptional heterogeneity and differential responses to immune pressure.Nature communications · 2024Article
- Antimicrobial peptide-based strategies to overcome antimicrobial resistance.Archives of microbiology · 2024Review
- Discovery of Biofilm Inhibitors from the Microbiota of Marine Egg Masses.Journal of natural products · 2024Article
- Oleanolic Acid Promotes the Formation of ProbioticMicroorganisms · 2024Article
- Deferiprone-Gallium-Protoporphyrin Chitogel DecreasesMaterials (Basel, Switzerland) · 2024Article
- Interactions of Neutrophils with the Polymeric Molecular Components of the Biofilm Matrix in the Context of Implant-Associated Bone and Joint Infections.International journal of molecular sciences · 2023Review
- Characterisation of Ferritin-Lymphocyte Ratio in COVID-19.Biomedicines · 2023Article
- Roles of the Crp/Fnr Family Regulator ArcR in the Hemolysis and Biofilm ofMicroorganisms · 2023Article
- The peanut root exudate increases the transport and metabolism of nutrients and enhances the plant growth-promoting effects of burkholderia pyrrocinia strain P10.BMC microbiology · 2023Article
- Potential additional effects of iron chelators on antimicrobial- impregnated central venous catheters.Frontiers in microbiology · 2023Article
- Bacterial biofilms in the human body: prevalence and impacts on health and disease.Frontiers in cellular and infection microbiology · 2023Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is a human pathogen that forms biofilm on catheters and medical implants. The authors' earlier study established that 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) inhibits biofilm formation by S. aureus by preventing the initial attachment of the cells to a solid surface and reducing the production of polysaccharide intercellular adhesin (PIA). Our cDNA microarray and MALDI-TOF mass spectrometric studies demonstrate that PGG treatment causes the expression of genes and proteins that are normally expressed under iron-limiting conditions. A chemical assay using ferrozine verifies that PGG is a strong iron chelator that depletes iron from the culture medium. This study finds that adding FeSO(4) to a medium that contains PGG restores the biofilm formation and the production of PIA by S. aureus SA113. The requirement of iron for biofilm formation by S. aureus SA113 can also be verified using a semi-defined medium, BM, that contains an iron chelating agent, 2, 2'-dipyridyl (2-DP). Similar to the effect of PGG, the addition of 2-DP to BM medium inhibits biofilm formation and adding FeSO(4) to BM medium that contains 2-DP restores biofilm formation. This study reveals an important mechanism of biofilm formation by S. aureus SA113.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.