ArticlePloS one2012
Identification of chromosomal genes in Yersinia pestis that influence type III secretion and delivery of Yops into target cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using β-Lactamase Fusions.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Illuminating Targets of Bacterial Secretion.PLoS pathogens · 2015Review
- Yersinia pestis targets neutrophils via complement receptor 3.Cellular microbiology · 2015Article
- Combinational deletion of three membrane protein-encoding genes highly attenuates yersinia pestis while retaining immunogenicity in a mouse model of pneumonic plague.Infection and immunity · 2015Article
- Resistance to Innate Immunity Contributes to Colonization of the Insect Gut by Yersinia pestis.PloS one · 2015Article
- RfaL is required for Yersinia pestis type III secretion and virulence.Infection and immunity · 2013Article
- Regulation of the Yersinia type III secretion system: traffic control.Frontiers in cellular and infection microbiology · 2013Review
- New functions for the ancient DedA membrane protein family.Journal of bacteriology · 2013Review
- YopK controls both rate and fidelity of Yop translocation.Molecular microbiology · 2013Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Pathogenic Yersinia species possess a type III secretion system, which is required for the delivery of effector Yop proteins into target cells during infection. Genes encoding the type III secretion machinery, its substrates, and several regulatory proteins all reside on a 70-Kb virulence plasmid. Genes encoded in the chromosome of yersiniae are thought to play important roles in bacterial perception of host environments and in the coordinated activation of the type III secretion pathway. Here, we investigate the contribution of chromosomal genes to the complex regulatory process controlling type III secretion in Yersinia pestis. Using transposon mutagenesis, we identified five chromosomal genes required for expression or secretion of Yops in laboratory media. Four out of the five chromosomal mutants were defective to various extents at injecting Yops into tissue culture cells. Interestingly, we found one mutant that was not able to secrete in vitro but was fully competent for injecting Yops into host cells, suggesting independent mechanisms for activation of the secretion apparatus. When tested in a mouse model of plague disease, three mutants were avirulent, whereas two strains were severely attenuated. Together these results demonstrate the importance of Y. pestis chromosomal genes in the proper function of type III secretion and in the pathogenesis of plague.
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