ArticlePloS one2012
Leishmania amazonensis arginase compartmentalization in the glycosome is important for parasite infectivity.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 99 citations in OpenAlex.
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- Caffeic Acid Phenethyl Ester (CAPE) Inhibits Arginase Activity and Growth ofPathogens (Basel, Switzerland) · 2025Article
- Polyamine Metabolism for Drug Intervention in Trypanosomatids.Pathogens (Basel, Switzerland) · 2024Review
- Early Leishmania infectivity depends on miR-372/373/520d family-mediated reprogramming of polyamines metabolism in THP-1-derived macrophages.Scientific reports · 2024Article
- Putrescine supplementation shifts macrophage L-arginine metabolism related-genes reducing Leishmania amazonensis infection.PloS one · 2023Article
- In Vitro and In Silico Analyses of New Cinnamid and Rosmarinic Acid-Derived Compounds Biosynthesized inPathogens (Basel, Switzerland) · 2022Article
- Polyamine Metabolism inMedical sciences (Basel, Switzerland) · 2022Review
- miR-294 and miR-410 Negatively RegulateNon-coding RNA · 2022Article
- Hide-and-Seek: A Game Played between Parasitic Protists and Their Hosts.Microorganisms · 2021Review
- Metabolomic Reprogramming of C57BL/6-Macrophages during Early Infection withInternational journal of molecular sciences · 2021Article
- The Effect of Naja naja oxiana Snake Venom Against Leishmania tropica Confirmed by Advanced Assays.Acta parasitologica · 2021Article
- Natural Products That Target the Arginase inMicroorganisms · 2021Review
- The Paradox of a Phagosomal Lifestyle: How Innate Host Cell-Frontiers in immunology · 2021Review
- SystematicFrontiers in chemistry · 2021Article
- Dual transcriptome analysis reveals differential gene expression modulation influenced byMicrobial genomics · 2020Article
- Role of Melatonin in the Synchronization of Asexual Forms in the ParasiteBiomolecules · 2020Review
- Differential expression of polyamine biosynthetic pathways in skin lesions and in plasma reveals distinct profiles in diffuse cutaneous leishmaniasis.Scientific reports · 2020Article
- Differential Regulation of l-Arginine Metabolism through Arginase 1 during Infection with Leishmania mexicana Isolates Obtained from Patients with Localized and Diffuse Cutaneous Leishmaniasis.Infection and immunity · 2020Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In Leishmania, de novo polyamine synthesis is initiated by the cleavage of L-arginine to urea and L-ornithine by the action of arginase (ARG, E.C. 3.5.3.1). Previous studies in L. major and L. mexicana showed that ARG is essential for in vitro growth in the absence of polyamines and needed for full infectivity in animal infections. The ARG protein is normally found within the parasite glycosome, and here we examined whether this localization is required for survival and infectivity. First, the localization of L. amazonensis ARG in the glycosome was confirmed in both the promastigote and amastigote stages. As in other species, arg(-) L. amazonensis required putrescine for growth and presented an attenuated infectivity. Restoration of a wild type ARG to the arg(-) mutant restored ARG expression, growth and infectivity. In contrast, restoration of a cytosol-targeted ARG lacking the glycosomal SKL targeting sequence (argΔSKL) restored growth but failed to restore infectivity. Further study showed that the ARGΔSKL protein was found in the cytosol as expected, but at very low levels. Our results indicate that the proper compartmentalization of L. amazonensis arginase in the glycosome is important for enzyme activity and optimal infectivity. Our conjecture is that parasite arginase participates in a complex equilibrium that defines the fate of L-arginine and that its proper subcellular location may be essential for this physiological orchestration.
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