ArticlePloS one2012
High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Assessment of MRI to estimate metastatic dissemination risk and prometastatic effects of chemotherapy.NPJ breast cancer · 2022Article
- Imaging Endocytosis Dynamics in Health and Disease.Membranes · 2022Review
- Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy.Bio-protocol · 2021Article
- Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose.Scientific reports · 2021Article
- Understanding the in vivo Fate of Advanced Materials by Imaging.Advanced functional materials · 2020Article
- Multiphoton intravital microscopy in small animals: motion artefact challenges and technical solutions.Journal of microscopy · 2020Review
- Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs.Methods (San Diego, Calif.) · 2017Article
- Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior.Advanced drug delivery reviews · 2017Review
- Article
- Fibre-coupled multiphoton microscope with adaptive motion compensation.Biomedical optics express · 2015Article
- New techniques for motion-artifact-free in vivo cardiac microscopy.Frontiers in physiology · 2015Review
- Imaging hallmarks of cancer in living mice.Nature reviews. Cancer · 2014Review
- Imaging the dynamics of endocytosis in live mammalian tissues.Cold Spring Harbor perspectives in biology · 2014Review
- Automated motion artifact removal for intravital microscopy, without a priori information.Scientific reports · 2014Article
- Advanced Motion Compensation Methods for Intravital Optical Microscopy.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society · 2014Article
- Imaging cell biology in live animals: ready for prime time.The Journal of cell biology · 2013Review
- From seeing to believing: labelling strategies for in vivo cell-tracking experiments.Interface focus · 2013Article
- Caught in the act: revealing the metastatic process by live imaging.Disease models & mechanisms · 2013Review
- Intravital microscopy: a practical guide on imaging intracellular structures in live animals.BioarchitectureReview
- IMART software for correction of motion artifacts in images collected in intravital microscopy.IntravitalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intravital imaging of brain and bone marrow cells in the skull with subcellular resolution has revolutionized neurobiology, immunology and hematology. However, the application of this powerful technology in studies of abdominal organs has long been impeded by organ motion caused by breathing and heartbeat. Here we describe for the first time a simple device designated 'microstage' that effectively reduces organ motions without causing tissue lesions. Combining this microstage device with an upright intravital laser scanning microscope equipped with a unique stick-type objective lens, the system enables subcellular-level imaging of abdominal organs in live mice. We demonstrate that this technique allows for the quantitative analysis of subcellular structures and gene expressions in cells, the tracking of intracellular processes in real-time as well as three-dimensional image construction in the pancreas and liver of the live mouse. As the aforementioned analyses based on subcellular imaging could be extended to other intraperitoneal organs, the technique should offer great potential for investigation of physiological and disease-specific events of abdominal organs. The microstage approach adds an exciting new technique to the in vivo imaging toolbox.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.