ArticlePloS one2012
Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae).
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 77 citations in OpenAlex.
- EsigPBP3 Was the Important Pheromone-Binding Protein to Recognize Male Pheromones and Key Eucalyptus Volatiles.International journal of molecular sciences · 2024Article
- Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes inInternational journal of molecular sciences · 2024Article
- Article
- An Expanded Survey of the Moth PBP/GOBP Clade inFrontiers in physiology · 2021Article
- Article
- Functional Analysis of Two Odorant-Binding Proteins, MaltOBP9 and MaltOBP10, inFrontiers in physiology · 2020Article
- Discrimination of Oviposition Deterrent Volatile β-Ionone by Odorant-Binding Proteins 1 and 4 in the WhiteflyBiomolecules · 2019Article
- Chemical Ecology ofFrontiers in physiology · 2019Article
- Functional Characteristics, Electrophysiological and Antennal Immunolocalization of General Odorant-Binding Protein 2 in Tea Geometrid, Ectropis obliqua.International journal of molecular sciences · 2018Article
- Molecular and Functional Characterization of Odorant Binding Protein 7 From the Oriental Fruit MothFrontiers in physiology · 2018Article
- Pheromone Binding Protein EhipPBP1 Is Highly Enriched in the Male Antennae of the Seabuckthorn Carpenterworm and Is Binding to Sex Pheromone Components.Frontiers in physiology · 2018Article
- Functional Analysis of the Chemosensory Protein MsepCSP8 From the Oriental ArmywormFrontiers in physiology · 2018Article
- Two Odorant-Binding Proteins of the Dark Black Chafer (Frontiers in physiology · 2018Article
- Structural Transformation Detection Contributes to Screening of Behaviorally Active Compounds: Dynamic Binding Process Analysis of DhelOBP21 from Dastarcus helophoroides.Journal of chemical ecology · 2017Article
- BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel).Scientific reports · 2017Article
- C-terminus Methionene Specifically Involved in Binding Corn Odorants to Odorant Binding Protein4 inFrontiers in physiology · 2017Article
- Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus.PloS one · 2017Article
- Identification and Characterization of Odorant Binding Proteins in the Forelegs ofFrontiers in physiology · 2017Article
- Article
- Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing.Frontiers in physiology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcription PCR and rapid amplification of cDNA ends. Recombinant LstiGOBP2 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR assays indicated that LstiGOBP2 mRNA is expressed mainly in adult antennae, with expression levels differing with developmental age. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the LstiGOBP2 protein has binding affinity to a broad range of odorants. Most importantly, trans-11-tetradecen-1-yl acetate, the pheromone component of Loxostege sticticalis, and trans-2-hexenal and cis-3-hexen-1-ol, the most abundant plant volatiles in essential oils extracted from host plants, had high binding affinities to LstiGOBP2 and elicited strong electrophysiological responses from the antennae of adults.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.