ArticlePloS one2012
A combined approach of high-throughput sequencing and degradome analysis reveals tissue specific expression of microRNAs and their targets in cucumber.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Profile and potential bioactivity of the miRNome and metabolome expressed in Malva sylvestris L. leaf and flower.BMC plant biology · 2023Article
- A methane-cGMP module positively influences adventitious rooting.Plant cell reports · 2023Article
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- Integrated High-Throughput Sequencing, Microarray Hybridization and Degradome Analysis Uncovers MicroRNA-Mediated Resistance Responses of Maize to PathogenInternational journal of molecular sciences · 2022Article
- Identification of Known and NovelLife (Basel, Switzerland) · 2022Article
- miRNA Profiling and Its Role in Multi-Omics Regulatory Networks Connected with Somaclonal Variation in Cucumber (International journal of molecular sciences · 2022Article
- Sequence, Secondary Structure, and Phylogenetic Conservation of MicroRNAs inBioinformatics and biology insights · 2022Article
- microRNA 166: an evolutionarily conserved stress biomarker in land plants targeting HD-ZIP family.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Waterlogging-Stress-Responsive LncRNAs, Their Regulatory Relationships with miRNAs and Target Genes in Cucumber (International journal of molecular sciences · 2021Article
- Characterization of microRNAs from neem (3 Biotech · 2021Article
- Article
- Profiling of MicroRNAs and Their Targets in Roots and Shoots Reveals a Potential MiRNA-Mediated Interaction Network in Response to Phosphate Deficiency in the Forestry TreeFrontiers in genetics · 2021Article
- Effects of Host-Adaptive Mutations on Hop Stunt Viroid Pathogenicity and Small RNA Biogenesis.International journal of molecular sciences · 2020Article
- Cross-Kingdom RNAi of Pathogen Effectors Leads to Quantitative Adult Plant Resistance in Wheat.Frontiers in plant science · 2020Article
- Article
- Identification of miRNAs and Their Target Genes Involved in Cucumber Fruit Expansion Using Small RNA and Degradome Sequencing.Biomolecules · 2019Article
- High-throughput sequencing reveals microRNAs in response to heat stress in the head kidney of rainbow trout (Oncorhynchus mykiss).Functional & integrative genomics · 2019Article
- Biology and Function of miR159 in Plants.Plants (Basel, Switzerland) · 2019Review
- Identification of cucumber circular RNAs responsive to salt stress.BMC plant biology · 2019Article
- Expression profiling and regulatory network of cucumber microRNAs and their putative target genes in response to cucumber green mottle mosaic virus infection.Archives of virology · 2019Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are endogenous small RNAs playing an important regulatory function in plant development and stress responses. Among them, some are evolutionally conserved in plant and others are only expressed in certain species, tissue or developmental stages. Cucumber is among the most important greenhouse species in the world, but only a limited number of miRNAs from cucumber have been identified and the experimental validation of the related miRNA targets is still lacking. In this study, two independent small RNA libraries from cucumber leaves and roots were constructed, respectively, and sequenced with the high-throughput Illumina Solexa system. Based on sequence similarity and hairpin structure prediction, a total of 29 known miRNA families and 2 novel miRNA families containing a total of 64 miRNA were identified. QRT-PCR analysis revealed that some of the cucumber miRNAs were preferentially expressed in certain tissues. With the recently developed 'high throughput degradome sequencing' approach, 21 target mRNAs of known miRNAs were identified for the first time in cucumber. These targets were associated with development, reactive oxygen species scavenging, signaling transduction and transcriptional regulation. Our study provides an overview of miRNA expression profile and interaction between miRNA and target, which will help further understanding of the important roles of miRNAs in cucumber plants.
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