ArticlePloS one2012
Development of an in vitro model for the multi-parametric quantification of the cellular interactions between Candida yeasts and phagocytes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Mucosal passive immunization with monoclonal antibodies targeting Candidalysin and Hyr proteins attenuates vaginal candidiasis in mice.Virulence · 2025Article
- Identification and Functional Analysis of GTP Cyclohydrolase II inFrontiers in microbiology · 2022Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
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- The isolation and identification of Candida glabrata from avian species and a study of the antibacterial activities of Chinese herbal medicine in vitro.Poultry science · 2021Article
- Article
- Vaccination with Secreted Aspartyl Proteinase 2 Protein fromInfection and immunity · 2020Article
- Divergent Approaches to Virulence inInternational journal of molecular sciences · 2019Review
- Candida auris: An emerging pathogen "incognito"?PLoS pathogens · 2019Article
- Microevolution of the pathogenic yeastsMicrobial cell (Graz, Austria) · 2019Review
- Hexokinase and Glucokinases Are Essential for Fitness and Virulence in the Pathogenic YeastFrontiers in microbiology · 2019Article
- TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Mechanisms involved in the triggering of neutrophil extracellular traps (NETs) by Candida glabrata during planktonic and biofilm growth.Scientific reports · 2017Article
- Fungal Strategies to Evade the Host Immune Recognition.Journal of fungi (Basel, Switzerland) · 2017Review
- Modelling the host-pathogen interactions of macrophages andJournal of the Royal Society, Interface · 2017Article
- Article
- Specific Human and Candida Cellular Interactions Lead to Controlled or Persistent Infection Outcomes during Granuloma-Like Formation.Infection and immunity · 2017Article
- Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells.Parasitology research · 2016Article
- Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood.Journal of visualized experiments : JoVE · 2016Article
- Disruption of Protein Mannosylation AffectsFrontiers in microbiology · 2016Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a new in vitro model for a multi-parameter characterization of the time course interaction of Candida fungal cells with J774 murine macrophages and human neutrophils, based on the use of combined microscopy, fluorometry, flow cytometry and viability assays. Using fluorochromes specific to phagocytes and yeasts, we could accurately quantify various parameters simultaneously in a single infection experiment: at the individual cell level, we measured the association of phagocytes to fungal cells and phagocyte survival, and monitored in parallel the overall phagocytosis process by measuring the part of ingested fungal cells among the total fungal biomass that changed over time. Candida albicans, C. glabrata, and C. lusitaniae were used as a proof of concept: they exhibited species-specific differences in their association rate with phagocytes. The fungal biomass uptaken by the phagocytes differed significantly according to the Candida species. The measure of the survival of fungal and immune cells during the interaction showed that C. albicans was the more aggressive yeast in vitro, destroying the vast majority of the phagocytes within five hours. All three species of Candida were able to survive and to escape macrophage phagocytosis either by the intraphagocytic yeast-to-hyphae transition (C. albicans) and the fungal cell multiplication until phagocytes burst (C. glabrata, C. lusitaniae), or by the avoidance of phagocytosis (C. lusitaniae). We demonstrated that our model was sensitive enough to quantify small variations of the parameters of the interaction. The method has been conceived to be amenable to the high-throughput screening of mutants in order to unravel the molecular mechanisms involved in the interaction between yeasts and host phagocytes.
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