Evidence map›Paper›PMID 22477469›Full record

ArticlePhotosynthesis research2012

Characterization of photosystem I from spinach: effect of solution pH.

Jianguo Liu, Xuefang Zhang, Meng Wang, Jing Liu, Meiwen Cao, Jianren Lu, Zhanfeng Cui

Abstract read
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Article in Photosynthesis research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jianguo LiuCenter for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, People's Republic of China. jianguoliu@upc.edu.cn
Xuefang Zhang
Meng Wang
Jing Liu
Meiwen Cao
Jianren Lu
Zhanfeng Cui

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our previous work has demonstrated the isolation of photosystem I (PSI) from spinach using ultrafiltration with a final purity of 84%. In order to get a higher purity of PSI and more importantly to develop a practical bioseparation process, key physiochemical properties of PSI and their dependence on operational parameters must be assessed. In this study, the effect of solution pH, one of the most important operating parameters for membrane process, on the property of PSI was examined. Following the isolation of crude PSI from spinach using n-dodecyl-beta-D: -maltoside as detergent, the isoelectric point, aggregation size, zeta potential, low-temperature fluorescence, atomic force microscopy imaging, secondary structure, and thermal stability were determined. Solution pH was found to have a significant effect on the activity, aggregation size and thermal stability of PSI. The results also suggested that the activity of PSI was related to its aggregation size.

Indexed as

Cell MembraneDetergentsFluorescenceGlucosidesHydrogen-Ion ConcentrationIsoelectric PointMicroscopy, Atomic ForcePhotosystem I Protein ComplexPlant ProteinsProtein StabilityProtein Structure, SecondarySolutionsSpinacia oleraceaTemperatureThylakoidsUltrafiltrationDetergentsdodecyl maltosideGlucosidesPhotosystem I Protein ComplexPlant ProteinsSolutions

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.