ArticleJournal of the Association for Research in Otolaryngology : JARO2012
Contractility in type III cochlear fibrocytes is dependent on non-muscle myosin II and intercellular gap junctional coupling.
Article in Journal of the Association for Research in Otolaryngology : JARO, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Macromolecular organization and fine structure of the human basilar membrane - RELEVANCE for cochlear implantation.Cell and tissue research · 2015Trial
- Electrical and Immunohistochemical Properties of Cochlear Fibrocytes in 3D Cell Culture and in the Excised Spiral Ligament of Mice.Journal of the Association for Research in Otolaryngology : JARO · 2022Article
- Delivery of therapeutics to the inner ear: The challenge of the blood-labyrinth barrier.Science translational medicine · 2019Review
- Forgotten Fibrocytes: A Neglected, Supporting Cell Type of the Cochlea With the Potential to be an Alternative Therapeutic Target in Hearing Loss.Frontiers in cellular neuroscience · 2019Article
- Article
- Computer modeling defines the system driving a constant current crucial for homeostasis in the mammalian cochlea by integrating unique ion transports.NPJ systems biology and applications · 2017Article
- The unique electrical properties in an extracellular fluid of the mammalian cochlea; their functional roles, homeostatic processes, and pathological significance.Pflugers Archiv : European journal of physiology · 2016Review
- Curcumin Reduces the Noise-Exposed Cochlear Fibroblasts Apoptosis.International archives of otorhinolaryngology · 2016Article
- The unique ion permeability profile of cochlear fibrocytes and its contribution to establishing their positive resting membrane potential.Pflugers Archiv : European journal of physiology · 2016Article
- Changes in Gene Expression and Hearing Thresholds After Cochlear Implantation.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2015Article
- NKCCs in the fibrocytes of the spiral ligament are silent on the unidirectional K⁺ transport that controls the electrochemical properties in the mammalian cochlea.Pflugers Archiv : European journal of physiology · 2015Article
- Connexins and gap junctions in the inner ear--it's not just about K⁺ recycling.Cell and tissue research · 2015Review
- Collagen matrix as a tool in studying fibroblastic cell behavior.Cell adhesion & migration · 2015Review
- Gap junctional coupling is essential for epithelial repair in the avian cochlea.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Panx1 regulates cellular properties of keratinocytes and dermal fibroblasts in skin development and wound healing.The Journal of investigative dermatology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The cochlear spiral ligament is a connective tissue that plays diverse roles in normal hearing. Spiral ligament fibrocytes are classified into functional sub-types that are proposed to carry out specialized roles in fluid homeostasis, the mediation of inflammatory responses to trauma, and the fine tuning of cochlear mechanics. We derived a secondary sub-culture from guinea pig spiral ligament, in which the cells expressed protein markers of type III or "tension" fibrocytes, including non-muscle myosin II (nmII), α-smooth muscle actin (αsma), vimentin, connexin43 (cx43), and aquaporin-1. The cells formed extensive stress fibers containing αsma, which were also associated intimately with nmII expression, and the cells displayed the mechanically contractile phenotype predicted by earlier modeling studies. cx43 immunofluorescence was evident within intercellular plaques, and the cells were coupled via dye-permeable gap junctions. Coupling was blocked by meclofenamic acid (MFA), an inhibitor of cx43-containing channels. The contraction of collagen lattice gels mediated by the cells could be prevented reversibly by blebbistatin, an inhibitor of nmII function. MFA also reduced the gel contraction, suggesting that intercellular coupling modulates contractility. The results demonstrate that these cells can impart nmII-dependent contractile force on a collagenous substrate, and support the hypothesis that type III fibrocytes regulate tension in the spiral ligament-basilar membrane complex, thereby determining auditory sensitivity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.