ArticleHistochemistry and cell biology2012
Fresh and cryopreserved amniotic membrane secrete the trefoil factor family peptide 3 that is well known to promote wound healing.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- Comparison of the effects of preservation methods on structural, biological, and mechanical properties of the human amniotic membrane for medical applications.Cell and tissue banking · 2024Review
- Application of Amniotic Membrane in Skin Regeneration.Pharmaceutics · 2023Review
- Comparison of human amniotic membrane decellularisation approaches for hESC-derived RPE cells culture.BMJ open ophthalmology · 2022Article
- Healing of a chronic ulcer of the lower limb of venous origin with fresh human amniochorionic membrane allograftBiomedica : revista del Instituto Nacional de Salud · 2022Article
- Amniotic membrane is a potential regenerative option for chronic non-healing wounds: a report of five cases receiving dehydrated human amnion/chorion membrane allograft.International wound journal · 2016Article
- Persistence of reduced expression of putative stem cell markers and slow wound healing in cultured diabetic limbal epithelial cells.Molecular vision · 2015Article
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
- A simple alkaline method for decellularizing human amniotic membrane for cell culture.PloS one · 2013Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amniotic membrane (AM) is often used for the treatment of ocular surface ulcerations and other corneal defects. Trefoil factor family (TFF) peptide 3 is produced by conjunctival goblet cells, participates in tear film physiology and has also been shown to be involved in ocular surface restitution after corneal injury. In the present study, we questioned whether AM also might be a source of TFF3 and if yes whether the secretion rate of TFF3 is changed by proinflammatory cytokines or by cryoconservation of AM. By means of RT-PCR, the mRNA expression of all three known TFF peptides could be detected in AM. Immunohistochemistry on paraffin-embedded sections localized TFF3 protein and also TFF2 in AM cells and Western blot analysis revealed TFF3 protein in AM. Stimulation experiments with proinflammatory cytokines and subsequent TFF3 ELISA measurements revealed that the secretion rate of fresh or cryoconserved AM was not significantly changed. The results indicate that TFF peptides are produced by AM. TFF3 may contribute to ocular surface wound healing after AM transplantation, but its production by AM is not further inducible by proinflammatory stimuli. Cryopreservation has no effect on the secretion rate of TFF3 supporting the use of cryopreserved AM for transplantation.
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