Evidence map›Paper›PMID 22476494›Full record

ArticleAoB PLANTS2011

A platform for efficient genotyping in Musa using microsatellite markers.

Pavla Christelová, Miroslav Valárik, Eva Hřibová, Ines Van den Houwe, Stéphanie Channelière, Nicolas Roux, Jaroslav Doležel

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Article in AoB PLANTS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Article
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  3. Diversity of Improved Diploids and Commercial Triploids fromCurrent issues in molecular biology · 2024
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  5. Striking variation in chromosome structure withinFrontiers in plant science · 2024
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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

7 authors.

Pavla ChristelováCentre of the Region Haná for Biotechnological and Agricultural Research , Institute of Experimental Botany , Sokolovská 6, Olomouc CZ-77200 , Czech Republic.
Miroslav Valárik
Eva Hřibová
Ines Van den Houwe
Stéphanie Channelière
Nicolas Roux
Jaroslav Doležel

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimsBananas and plantains (Musa spp.) are one of the major fruit crops worldwide with acknowledged importance as a staple food for millions of people. The rich genetic diversity of this crop is, however, endangered by diseases, adverse environmental conditions and changed farming practices, and the need for its characterization and preservation is urgent. With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers. METHODOLOGY: The genotyping system is based on 19 microsatellite loci, which are scored using fluorescently labelled primers and high-throughput capillary electrophoresis separation with high resolution. This genotyping platform was tested and optimized on a set of 70 diploid and 38 triploid banana accessions. PRINCIPAL

resultsThe marker set used in this study provided enough polymorphism to discriminate between individual species, subspecies and subgroups of all accessions of Musa. Likewise, the capability of identifying duplicate samples was confirmed. Based on the results of a blind test, the genotyping system was confirmed to be suitable for characterization of unknown accessions.

conclusionsHere we report on the first complex and standardized platform for molecular characterization of Musa germplasm that is ready to use for the wider Musa research and breeding community. We believe that this genotyping system offers a versatile tool that can accommodate all possible requirements for characterizing Musa diversity, and is economical for samples ranging from one to many accessions.

Identifiers

PMID22476494
PMCPMC3185971

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.