ReviewBioelectrochemistry (Amsterdam, Netherlands)2012
A brief overview of electroporation pulse strength-duration space: a region where additional intracellular effects are expected.
Review in Bioelectrochemistry (Amsterdam, Netherlands), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
97 citing papers in PubMed, 273 citations in OpenAlex.
- Pulsed Field Ablation Therapy Parameters: Enhancing Lesion Depth While Reducing Musculoskeletal Stimulation and Thermal Response in an Irrigated Tip Catheter: A Comprehensive Study Using In Silico, In Vitro, and In Vivo Models.Circulation. Arrhythmia and electrophysiology · 2026Article
- High Intensity Microsecond-Electric Pulses Modulate Cell Death in MDA-MB-231 Cells Treated with Resveratrol.Cell biochemistry and biophysics · 2026Article
- Production of Large Ellipsoidal Ablations Using Integrated Nanosecond Pulse Irreversible Electroporation Administered via a Single Applicator and Grounding Pad.Journal of vascular and interventional radiology : JVIR · 2026Article
- A critical review of microfluidic electroporation for therapeutic cell engineering.Cell reports. Physical science · 2026Article
- Regulation of Pore Evolution via Progressive Electroporation Enhanced Intracellular Molecule Transport.Research (Washington, D.C.) · 2026Article
- Low-cost, handheld, multi-pulse electroporators for simplified nucleic acid delivery in skin.Bioengineering & translational medicine · 2026Article
- Combined Locally Enhanced Electric Field Treatment and Copper for Effective Inactivation of Gram-Positive and Gram-Negative Bacteria in Water.ACS ES&T engineering · 2025Article
- Effects of ultra-fast nanosecond electric pulses on mitochondria transmembrane potential and oxidation.Scientific reports · 2025Article
- Impact of Voltage on the Production on Spherical Ablations for Integrated Time Nanosecond Pulse Irreversible Electroporation.Bioelectricity · 2025Article
- Article
- High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds.Journal of visualized experiments : JoVE · 2025Article
- Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells.Biological research · 2025Article
- Inkjet-printed transparent electrodes: Design, characterization, and initial in vivo evaluation for brain stimulation.PloS one · 2025Article
- Article
- Complexity and Perplexity of Pulsed Field Ablation: An Engineering Perspective.Arrhythmia & electrophysiology review · 2025Review
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
- A hybridized mechano-electroporation technique for efficient immune cell engineering.Journal of advanced research · 2024Article
- An electroporation cytometry system for long-term, live cell cycle analysis.Biomicrofluidics · 2024Article
- Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Integrated Time Nanosecond Pulse Irreversible Electroporation (INSPIRE): Assessment of Dose, Temperature, and Voltage on Experimental and Clinical Treatment Outcomes.IEEE transactions on bio-medical engineering · 2024Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Electroporation (EP) of outer cell membranes is widely used in research, biotechnology and medicine. Now intracellular effects by organelle EP are of growing interest, mainly due to nanosecond pulsed electric fields (nsPEF). For perspective, here we provide an approximate overview of EP pulse strength-duration space. This overview locates approximately some known effects and applications in strength-duration space, and includes a region where additional intracellular EP effects are expected. A feature of intracellular EP is direct, electrical redistribution of endogenous biochemicals among cellular compartments. For example, intracellular EP may initiate a multistep process for apoptosis. In this hypothesis, initial EP pulses release calcium from the endoplasmic reticulum, followed by calcium redistribution within the cytoplasm. With further EP pulses calcium penetrates mitochondrial membranes and causes changes that trigger release of cytochrome c and other death molecules. Apoptosis may therefore occur even in the presence of apoptotic inhibitors, using pulses that are smaller, but longer, than nsPEF.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.