ArticleNeural development2012
Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain.
Article in Neural development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
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- Zebrafish Tools for Deciphering Habenular Network-Linked Mental Disorders.Biomolecules · 2021Review
- T-Cell Factors as Transcriptional Inhibitors: Activities and Regulations in Vertebrate Head Development.Frontiers in cell and developmental biology · 2021Review
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- Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo.Histochemistry and cell biology · 2020Review
- Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish.Histochemistry and cell biology · 2020Article
- Protein-Protein interaction network analyses of human WNT proteins involved in neural development.Bioinformation · 2019Article
- Canonical Wnt signaling regulates patterning, differentiation and nucleogenesis in mouse hypothalamus and prethalamus.Developmental biology · 2018Article
- Article
- Maternal exposure to prostaglandin EBiochemistry and biophysics reports · 2018Article
- An Eye on the Wnt Inhibitory Factor Wif1.Frontiers in cell and developmental biology · 2018Review
- An Evolutionarily Conserved Network Mediates Development of the zona limitans intrathalamica, a Sonic Hedgehog-Secreting Caudal Forebrain Signaling Center.Journal of developmental biology · 2016Review
- Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells.BMC genomics · 2016Article
- Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain.Frontiers in neuroanatomy · 2014Article
- The ventral habenulae of zebrafish develop in prosomere 2 dependent on Tcf7l2 function.Neural development · 2013Article
- Ciliogenesis and cerebrospinal fluid flow in the developing Xenopus brain are regulated by foxj1.Cilia · 2013Article
- Neurogenesis in zebrafish - from embryo to adult.Neural development · 2013Review
- Wnt signaling in vertebrate neural development and function.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2012Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundA fundamental requirement for development of diverse brain regions is the function of local organizers at morphological boundaries. These organizers are restricted groups of cells that secrete signaling molecules, which in turn regulate the fate of the adjacent neural tissue. The thalamus is located in the caudal diencephalon and is the central relay station between the sense organs and higher brain areas. The mid-diencephalic organizer (MDO) orchestrates the development of the thalamus by releasing secreted signaling molecules such as Shh.
resultsHere we show that canonical Wnt signaling in the caudal forebrain is required for the formation of the Shh-secreting MD organizer in zebrafish. Wnt signaling induces the MDO in a narrow time window of 4 hours - between 10 and 14 hours post fertilization. Loss of Wnt3 and Wnt3a prevents induction of the MDO, a phenotype also observed upon blockage of canonical Wnt signaling per se. Pharmaceutical activation of the canonical Wnt pathways in Wnt3/Wnt3a compound morphant embryos is able to restore the lack of the MDO. After blockage of Wnt signaling or knock-down of Wnt3/Wnt3a we find an increase of apoptotic cells specifically within the organizer primordium. Consistently, blockage of apoptosis restores the thalamus organizer MDO in Wnt deficient embryos.
conclusionWe have identified canonical Wnt signaling as a novel pathway, that is required for proper formation of the MDO and consequently for the development of the major relay station of the brain - the thalamus. We propose that Wnt ligands are necessary to maintain the primordial tissue of the organizer during somitogenesis by suppressing Tp53-mediated apoptosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.