ArticleProceedings of the National Academy of Sciences of the United States of America1990
Developmentally regulated expression of an exon containing a stop codon in the gene for glutamic acid decarboxylase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 83 citations in OpenAlex.
- BDNF and JNK Signaling Modulate Cortical Interneuron and Perineuronal Net Development: Implications for Schizophrenia-Linked 16p11.2 Duplication Syndrome.Schizophrenia bulletin · 2021Article
- A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme.Scientific reports · 2018Article
- GAD1 alternative transcripts and DNA methylation in human prefrontal cortex and hippocampus in brain development, schizophrenia.Molecular psychiatry · 2018Article
- Glutamic acid decarboxylase 1 alternative splicing isoforms: characterization, expression and quantification in the mouse brain.BMC neuroscience · 2014Article
- Expression of GABA signaling molecules KCC2, NKCC1, and GAD1 in cortical development and schizophrenia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Dynamic regulation of glutamate decarboxylase 67 gene expression by alternative promoters and splicing during rat testis maturation.Molecular biology reports · 2010Article
- Utilization of an intron located polyadenlyation site resulted in four novel glutamate decarboxylase transcripts.Molecular biology reports · 2009Article
- Adult and embryonic GAD transcripts are spatiotemporally regulated during postnatal development in the rat brain.PloS one · 2009Article
- SDF1alpha/CXCR4 signaling, via ERKs and the transcription factor Egr1, induces expression of a 67-kDa form of glutamic acid decarboxylase in embryonic hippocampal neurons.The Journal of biological chemistry · 2008Article
- Post-translational regulation of L-glutamic acid decarboxylase in the brain.Neurochemical research · 2008Review
- Comparison of changes in GAD65 and GAD67 immunoreactivity and levels in the gerbil main olfactory bulb induced by transient ischemia.Neurochemical research · 2008Article
- Expression of an intron-containing beta-tubulin mRNA in catfish olfactory epithelium.Journal of molecular histology · 2007Article
- Expression of GAD67 and novel GAD67 splice variants during human fetal pancreas development: GAD67 expression in the fetal pancreas.Endocrine pathology · 2007Article
- Article
- A genomic overview of pyridoxal-phosphate-dependent enzymes.EMBO reports · 2003Review
- Dynamic expression of a glutamate decarboxylase gene in multiple non-neural tissues during mouse development.BMC developmental biology · 2001Article
- Mutations in exon 3 of the glycogen debranching enzyme gene are associated with glycogen storage disease type III that is differentially expressed in liver and muscle.The Journal of clinical investigation · 1996Article
- Differentiation of the immortalized adult neuronal progenitor cell line HC2S2 into neurons by regulatable suppression of the v-myc oncogene.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Distinct protein forms are produced from alternatively spliced bicistronic glutamic acid decarboxylase mRNAs during development.Molecular and cellular biology · 1994Article
- Convulsions and inhibition of glutamate decarboxylase by pyridoxal phosphate-gamma-glutamyl hydrazone in the developing rat.Neurochemical research · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In the adult rat brain, the gene for glutamic acid decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.1.15) is expressed predominantly as a 3.7-kilobase transcript. Earlier data showed that embryonic brain expresses an RNA transcript distinct from the adult form; however, the exact structure of this form was not elucidated. Here, transcripts expressed in the embryonic but not the adult brain were cloned and analyzed. These transcripts include an exon not expressed in the adult inserted into coding sequence. The embryonic exon contains a stop codon that is in-frame with the coding sequence. The exon is found in genomic DNA within the GAD gene where it is flanked by introns with conventional splice sites. On the basis of these structural data, we propose the hypothesis that, early in brain development, transcripts encoding a truncated form of GAD are expressed. The deduced protein cannot function as a decarboxylase because the stop codon in the embryonic exon occurs upstream of the binding site for pyridoxal phosphate, an essential cofactor. Thus, alternative splicing plays a crucial role in the pathway leading to the development of functional GABAergic neurons. The central nervous system-derived cell lines B65 and C6 express a mixture of the adult and embryonic forms of GAD mRNA. They therefore are useful clonal models of central nervous system cells in the early phases of differentiation.
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